RapiZyme™ Trypsin, Mass Spectrometry (MS) Grade yields quicker, cleaner, more complete, and easily interpretable protein digestions. RapiZyme trypsin is novel in that it is extensively dimethylated at lysine residues leading to inordinately high resistance to autolysis. RapiZyme trypsin can be used at comparatively high concentrations to achieve faster digestions without problematic contaminating autolysis peaks.
RapiZyme trypsin is a chemically optimized recombinant trypsin. Because it is not animal derived, it is free of contaminating chymotrypsin, does not require treatment with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) inhibitors, and thus is free from non-specific digestion associated challenges. RapiZyme trypsin is provided in 100 µg quantities and is lyophilized with an LC-MS compatible additive to improve its stability and vial recovery while maintaining high activity upon reconstitution.
Overall, robust activity, stability and selectivity makes RapiZyme trypsin an industry-leading protease available for high quality protein digestions.
Detection Method |
MS, Optical, UV |
Formulation |
Solid |
Storage Condition |
-20C +/- 10C (Freezer) |
Instrument |
UPLC, UHPLC, HPLC |
Shelf Life |
24 Months |
Shelf life upon reconstitution |
1 Month |
Shipping Condition |
Cold Chain |
Mass |
100 µg/vial |
UNSPSC |
41116105 |
Application |
Protein Digestion |
Brand |
RapiZyme |
Product Type |
Reagents |
Units per Package |
4 pk |
RapiZyme Trypsin, MS Grade, 4/pk is a high-quality, mass spectrometry-grade enzyme preparation specifically designed to facilitate efficient and reproducible protein digestion. This product is made for proteomic research, where precise and consistent results are paramount.
RapiZyme Trypsin is sourced from bovine pancreas and undergoes a rigorous purification process to ensure the highest levels of purity and activity. This ensures that the enzyme is free from contaminants that could interfere with mass spectrometry analysis, such as autolytic fragments or other proteases. The result is a trypsin preparation that delivers reliable and reproducible cleavage of peptide bonds at the carboxyl side of lysine and arginine residues, generating peptides that are ideal for mass spectrometry identification and quantification.
One of the standout features of RapiZyme Trypsin, MS Grade, is its stability and activity over a wide range of conditions. The enzyme is lyophilized to enhance its shelf life and stability, ensuring that it retains its activity even after extended storage. This makes it an ideal choice for laboratories that require a reliable enzyme that can be stored and used over a long period without degradation. When reconstituted, RapiZyme Trypsin maintains its activity, providing consistent results across different experiments and conditions.
The use of RapiZyme Trypsin in proteomic workflows enhances the efficiency of protein digestion, reducing the time required for sample preparation. Its high specific activity allows for rapid and complete digestion of proteins, minimizing the risk of incomplete digestion and ensuring that the resulting peptides are suitable for downstream mass spectrometry analysis. This efficiency is crucial for high-throughput laboratories that need to process large numbers of samples quickly and accurately.
RapiZyme Trypsin, MS Grade, also offers excellent reproducibility between batches, thanks to the stringent quality control processes Waters uses. Each batch of trypsin is tested to ensure that it meets the highest standards of purity, activity, and performance. This consistency between batches ensures that researchers can rely on RapiZyme Trypsin for their critical proteomic experiments, reducing variability and enhancing the reliability of their results.
In addition to its high performance, RapiZyme Trypsin is easy to use. Each vial contains a precisely measured amount of enzyme, simplifying the preparation of digestion reactions. Researchers can quickly and easily reconstitute the enzyme with water or buffer, streamlining the workflow and reducing the potential for errors. The convenience of the 4/pk format allows laboratories to manage their enzyme inventory efficiently, ensuring that they always have a fresh supply of trypsin available for their experiments.
RapiZyme Trypsin, MS Grade, 4/pk, is a valuable addition to any proteomic research laboratory. Its high purity, stability, and activity make it an ideal choice for protein digestion in mass spectrometry workflows. By providing reliable and reproducible results, RapiZyme Trypsin helps researchers achieve their experimental goals with confidence and efficiency. Whether you are conducting routine analyses or complex proteomic studies, RapiZyme Trypsin delivers the performance and reliability you need to succeed.
FAQs About RapiZyme Trypsin, MS Grade, 4/pk
1.What are the recommended storage conditions for RapiZyme Trypsin, MS Grade?
RapiZyme Trypsin is lyophilized to enhance its shelf life and stability. It should be stored in a cool, dry place. Once reconstituted, it is recommended to store the enzyme at -20°C for long-term use to maintain its activity.
2.Can RapiZyme Trypsin, MS Grade, be used for high-throughput applications?
Yes, RapiZyme Trypsin is well-suited for high-throughput applications. Its high specific activity allows for rapid and complete digestion of proteins, making it ideal for laboratories that need to process large numbers of samples efficiently.
3.How is RapiZyme Trypsin, MS Grade, prepared?
RapiZyme Trypsin is sourced from bovine pancreas and undergoes a rigorous purification process to ensure high purity and activity. This preparation process removes contaminants that could interfere with mass spectrometry analysis and meets Waters" rigorous standards.