ACQUITY UPLC Columns
Increase speed, sensitivity, and resolution, without compromising data integrity
Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.
Waters ACQUITY UPLC Columns’ sub-2-μm particle chemistries enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability.
ACQUITY UPLC Columns
Mitigate unwanted interactions between analytes and metal ions associated with stainless-steel column hardware with ACQUITY Premier Columns.
Specifications
Overview
- Best-in-class column efficiency for superior separation speed, sensitivity, and resolution
- 8 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
- 22 stationary phases, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP
- 7 application-specific UPLC Column chemistries for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis
- More than 50 different column dimensions and configurations, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm
- Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using ACQUITY stationary phases
Recommended Use: For improved speed, sensitivity, and resolution in methods development.
ACQUITY UPLC BEH Column Characteristics
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Universal C18 column
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Suitable for a diverse range of analytes
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High efficiency
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Industry-leading mobile phase pH 1-12 and temperature compatibility
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Lower hydrophobicity and retention than a C18 column
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Suitable for a diverse range of analytes
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High efficiency
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Industry-leading mobile phase pH 1-12 and temperature compatibility
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Contains an embedded polar group combined with the hydrophobicity of C18
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Complementary selectivity to a C18 column
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Enhanced peak shape for basic compounds
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Compatible with 100% aqueous mobile phases
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High efficiency
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Industry leading chemical stability
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Suitable for a diverse range of analytes
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High efficiency
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Complimentary selectivity to straight-chain alkyl phases
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Improves the retention of very polar basic analytes
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Complementary selectivity to reversed-phase
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High efficiency
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Exceptional chemical stability and peak shape compared to silica-based HILIC stationary phases
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Designed to retain highly polar analytes that are too polar to retain by reversed-phase
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Exceptional chemical stability at high pH and high temperature
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Ideal for sugar/carbohydrate analysis
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Exceptional retention of polar analytes spanning a wide range of polarity
ACQUITY UPLC CSH Column Characteristics
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Universal column choice for UV and MS applications
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Suitable for a broad range of compounds
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Alternate selectivity to BEH C18
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Exceptional peak shape and increased load capacity
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Suited for basic compounds at low pH
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Exceptional selectivity for positional isomers, halogenated compounds and polar compounds
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Enhanced retention of acidic compounds compared to traditional PFP-bonded stationary phases
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Complementary selectivity to straight-chain alkyl phases
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Exceptional peak shape for basic compounds under low- and high-pH conditions
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Increased retention for acidic compounds at low pH
ACQUITY UPLC HSS Column Characteristics
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Ideal for the enhanced retention of polar and non-polar compounds
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Compatible with 100% aqueous mobile phases
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Mechanically stable at elevated pressures
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General purpose silica-based C18 chemistry
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Suitable for a broad range of compounds
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Provides exceptional peak shapes and low pH stability
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Delivers increased retention compared to hybrid organic/inorganic C18 columns
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Low coverage silica-based C18 chemistry
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Provides alternate selectivity for compounds impacted by silanophilic interactions
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Increased silanol activity results in greater retention of basic compounds while reducing the retention of non-basic analytes
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Provides exceptional peak shape and imparts Selectivity for Bases at low pH (SB)
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Pentafluorophenyl (PFP) chemistry
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Industry-leading reproducibility and peak shape
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Ideally suited for planar aromatic compounds, positional isomers, halogenated compounds and polar analytes
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Provides low hydrophobicity and unique selectivity compared to straight-chain-alkyl columns
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Ultra-stable retention
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Exceptional peak shape and reproducibility under low- to mid-pH conditions
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Compatible with both reversed-phase and normal-phase techniques
ACQUITY UPLC Application-Specific Column Characteristics
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Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
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Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns
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Two pore sizes (130 Å and 300 Å) for a wide range of peptides
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Outstanding peak capacities with formic acid for MS-based applications
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Excellent performance with TFA for optical based applications
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Less dependence on TFA or FA to achieve optimal peak capacity
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Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities
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At low pH with the positive charge on CSH, you get less secondary interactions with the particle
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Separations that require silica-based selectivity
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Separations that necessitate increased peptide retention
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Ideally suited for small, hydrophilic peptides
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Sub-2-μm particle columns for UPLC separations (2.5 μm for 450 Å)
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Pore size options include: 125 Å, 200 Å, and 450 Å
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Specifically designed and QC tested with BEH125, BEH200, or BEH450 protein standards to help ensure batch-to-batch consistency
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10x faster than traditional HPLC-based SEC
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Separates proteins of various sizes, hydrophobicities, and isoelectric points
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Tolerates extreme pH and temperature, and provides minimal secondary interactions
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Improves sensitivity for phosphorylated proteins and low-level intact and subunit mAb analysis
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High efficiency separations with 1.7 µm particles
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Suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase chromatography
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Suited for the HILIC analysis of neutral and acidic glycans
Get superior peak shape for basic analytes
Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.
The ruggedness you want, the repeatability you need
ACQUITY UPLC BEH 1.7 μm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.
Achieve flexibility in method development
Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.
