The development and successful commercialization of protein-based biopharmaceuticals and diagnostic reagents frequently depends on the ability to adequately characterize these complex biomolecules. Waters ACQUITY^® XBridge™ BEH300 C₄ reversed-phase columns and associated methods can help improve your protein separation and characterization challenges.

XBridge BEH300 C4 Reversed-Phase HPLC Columns
Wide pore, Ethyl Bridged Hybrid (BEH) based 3.5 um particles with C4 ligand well suited for HPLC-based protein and polypeptide characterization.

Waters is a world leader in the research, development, and commercialization of C₁₈, reversed-phase columns for many applications. However, the separation of large molecular weight biological compounds can be challenging and be significantly improved by changing the physical properties of the column packing material. For more than 15 years, chromatographers have benefited from performance characteristics that Waters reversed-phase columns have provided for protein separations. Now see how Waters Ethyl Bridged Hybrid (BEH) Particle Technology can help overcome performance limitations experienced using 100% silica-based materials for challenging protein separations.

• 300Å pores
• C₄ ligand
• Minimal undesirable secondary interactions
• Minimal detectable carryover at elevated temperature separations

 300Å, C₄ Columns for Protein Chromatography

The separation of a standard protein mixture shows that the BEH300 C₄ column can be used with proteins representing a wide range of properties. This sample includes proteins with high and low isoelectric points, molecular weights from 12-94 kDa, and that elute anywhere from 20-55% acetonitrile.

Waters invests significant research and development time to deliver solutions that help organizations address today's separation challenges. As a primary manufacturer of separations media, our ability to consistently deliver reliable, quality products, and support is second to none in the industry. Discover how our BEH300 C₄ columns supported by our world-class R&D, applications, and distribution organizations, help make the "Science of What's Possible" a reality.

• Well characterized, state-of-the-art bonding procedures
• Low pH and high temperature stability
• Quality-control tested with diverse protein mixture
• Consistent batch-to-batch reproducibility
• Available 1.7 μm particles for UPLC and nano UPLC applications

 BEH300 C₄ vs. Competitive Column Stability Under Protein Separation Conditions

Overlay of 6 injections on XBridge^TM BEH300 C₄ vs. competitive 100% silica, 300Å C₄ column. Column stored with 0.1% TFA at 90 °C between protein sample injections at times 0, 5, 10, 40 and 60 hours.