The aim of this course is to study the fragmentation of positively and negatively charged even-electron ions of small molecules during in-source CID or MS/MS in triple quadrupole, ion trap, and quadrupole time-of-flight hybrid systems. The emphasis is on spectrum interpretation rather than on fragmentation mechanisms. In addition to lectures and ample time for questions and discussion, the course provides exercises in order to practice spectral interpretation.
Topics Covered
Information from mass spectra: Average and monoisotopic mass, mass defects, double-bond equivalents, the nitrogen rule, isotope peaks, adduct ions and adduct-bound dimers
Fragmentation techniques: Collision-induced dissociation (CID), In-source CID, Triple quadrupole, ion trap and quadrupole-time-of-flight (QTof) hybrid instruments.
General aspects of fragmentation: Basic fragmentation mechanisms as well as the ability to discuss fragmentation as charge-site-directed process are investigated.
Fragmentation of even-electron positive ions 1: Oxygen compounds -1: Alcohols, ethers, glycosides, aldehydes, ketones, flavonoids, and steroids.
Fragmentation of even-electron positive ions 2: Oxygen and sulphur compounds -2: Carboxylic acids, esters, triacylglycerols, as well as various sulfur compounds, e.g., sulfonamide antibiotics.
Fragmentation of even-electron positive ions 3: Nitrogen compounds -1: Amines, cyclic amines, quaternary ammonium compounds, and aromatic nitrogen compounds like triazines.
Fragmentation of even-electron positive ions 4: Nitrogen compounds -2: Amides, carbamates, organophosphorous compounds, halogenides, nitro-compounds, nitrosamines, and some aromatic heterocyclic ring compounds.
Strategies for confirmation and identification using LC/MS/MS.
Who Should Attend
Anyone that wants to learn how to interpret mass spectra
Prerequisites
At least 6 months experience with LC/MS
Course Notes
Each attendee will receive a course manual to keep for future reference and a Certificate of Attendance.
Contact Information
Questions? In the UK, call
Mike Stace at 0208 238 6100.
Cancellation Policy
Customer cancellations will carry a surcharge. Courses are run with small numbers
for maximum effect. Cancellation/transfers within 3-4 weeks of the course start
date will incur a charge of 20% of the course fee, 2-3 weeks prior, 50% of
the course fee, 1 week prior, 100% of course fee.
- Travel arrangements should
be made upon receipt of confirmation only.
- Waters is not responsible for
reimbursement of non-refundable tickets as a result of class cancellation
by Waters Corporation.
- Acceptance of the registration for this course indicates
that Waters Corporation will not be held responsible for any inconvenience
or losses incurred during the training or while traveling for this course.
Waters Corporation reserves the right to cancel any course 10 business days
prior to the course's start date, when minimum enrolment is not met.
Hours
Course times Monday to Thursday, 9:30 am to 5:00 pm; Friday 9:30 am to 2:00 pm.
For travel information for our Wilmslow facility, click here.
Elstree
Waters Ltd.
610 Centennial Park
Centennial Avenue
Elstree
Herts
WD6 3TJ
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eMail: uk@waters.com
The Waters UK headquarters is located adjacent to M1 junction 4, and is within easy reach of central London and is served by two airports, London Heathrow (28 miles) and London Luton (21 miles).
By Air
- London Heathrow Airport
Flight Information Tel: +44 (0) 870 0000123
- London Luton Airport
Main Switchboard Tel: +44 (0) 1582 405100. A free shuttle bus takes passengers on the 1.5 miles journey from Luton Parkway Station to the terminal.
By Rail
By Taxi
- Borehamwood Radio Cars
Tel: 0208 207 2288
- Jubilee Cars
Tel: 0208 950 8000
Wilmslow, Cheshire
The Corporate Centre for MS Technology Research, Development and Manufacturing
Waters Wilmslow
Stamford Avenue
Altrincham Road
Wilmslow
SK9 4AX
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eMail: uk@waters.com
Waters Wilmslow is situated minutes away from the most accessible international airport in the UK, gateway to 180 destinations worldwide. Manchester Airport also has regular shuttle feeder flights to London and EU gateways
By Air
By Rail
Accommodation
- We book accommodation on your behalf at a local hotel. Hotel accounts are payable in full on departure.
