Topics Covered
UPLC theory & chemistry
- UPLC principle & theory
- Resolution & peak capacity
- UPLC column chemistries
- Column design
- System bandspreading
ACQUITY hardware
- Solvent manager
- Degasser
- Sample manager
- Column manager
- eCord technology
- Detectors
ACQUITY console software
- Overview of console software
- Module functions and diagnostics
- Interactive displays
- Plots and logs
- Preparing for analysis
- Characterizing needle and loop volume
- Instrument method parameters
Method transfer, HPLC to UPLC
- Method transfer considerations
- Transfer of isocratic methods
- Transfer of gradient methods
- System delay volume
- Using the ACQUITY column calculator
Troubleshooting tips and tricks
- Mobile phase preparation
- Controlling bacterial growth
- Column maintenance
- Tips and tricks for the solvent and sample manager
- Optimal detector settings
- Troubleshooting peak shape problems and carry over
Hands-on exercises
Who Should Attend
The course is designed for users who currently use the ACQUITY UPLC H-Class Bio System. For the user who is involved in Instrument Operation and for those whose responsibilities include Method Transfer and/or Method Development
Prerequisites
Attendees should have the ACQUITY UPLC H-Class Bio Systems installed in their lab. A working knowledge of Empower 2 or MassLynx is strongly recommended.
Course Notes
Each attendee will receive a course manual to keep for future reference and a Certificate of Attendance
Cancellation Policy
Courses will have a minimum of 4 attendees. Attendees should have a minimum of 6 weeks experience operating the equipment. While every effort will be made to accommodate customer training requirements, attendees should note that training may be performed on a different version of hardware/software than that which is being used in their lab. Cancellation is required 10 business days prior to the start of a course to avoid 100% billing.
Hours
Class times may vary by course but generally are 9:00 am - 5:00 pm. Lunch and refreshments are included in the course fee.
Waters Chromatography Ireland Limited,
3rd Floor J5 Plaza
North Park Business Park
North Road
Finglas
Dublin 11
Directions from Airport
Take the M1 to City Centre/Port
Follow signs for Southbound/M50
Continue on M50 Southbound
Exit At junction 5, Use the left lane to take the N2 exit to City Centre/Finglas
At the first Lights turn right and then right at the first building J5 plaza.
Directions from M50
Exit At junction 5, Use the left lane to take the N2 exit to City Centre/Finglas
At the first Lights turn right and then right at the first building J5 plaza.
