Topics Covered
UPLC theory & chemistry
- UPLC principle & theory
- Resolution & peak capacity
- UPLC column chemistries
- Column design
- System bandspreading
ACQUITY hardware
- Solvent manager
- Degasser
- Sample manager
- Column manager
- eCord technology
- Detectors
ACQUITY console software
- Overview of console software
- Module functions and diagnostics
- Interactive displays
- Plots and logs
- Preparing for analysis
- Characterizing needle and loop volume
- Instrument method parameters
Method transfer, HPLC to UPLC
- Method transfer considerations
- Transfer of isocratic methods
- Transfer of gradient methods
- System delay volume
- Using the ACQUITY column calculator
Troubleshooting tips and tricks
- Mobile phase preparation
- Controlling bacterial growth
- Column maintenance
- Tips and tricks for the solvent and sample manager
- Optimal detector settings
- Troubleshooting peak shape problems and carry over
Hands-on exercises
Who Should Attend
The course is designed for users who currently use the ACQUITY UPLC H-Class Bio System. For the user who is involved in Instrument Operation and for those whose responsibilities include Method Transfer and/or Method Development
Prerequisites
Attendees should have the ACQUITY UPLC H-Class Bio Systems installed in their lab. A working knowledge of Empower 2 or MassLynx is strongly recommended
Course Notes
Each attendee will receive a course manual to keep for future reference and a Certificate of Attendance
Contact Information
Questions? In the UK, call
Mike Stace at 0208 238 6100.
Cancellation Policy
Customer cancellations will carry a surcharge. Courses are run with small numbers
for maximum effect. Cancellation/transfers within 3-4 weeks of the course start
date will incur a charge of 20% of the course fee, 2-3 weeks prior, 50% of
the course fee, 1 week prior, 100% of course fee.
- Travel arrangements should
be made upon receipt of confirmation only.
- Waters is not responsible for
reimbursement of non-refundable tickets as a result of class cancellation
by Waters Corporation.
- Acceptance of the registration for this course indicates
that Waters Corporation will not be held responsible for any inconvenience
or losses incurred during the training or while traveling for this course.
Waters Corporation reserves the right to cancel any course 10 business days
prior to the course's start date, when minimum enrolment is not met.
Hours
Course times Monday to Thursday, 9:30 am to 5:00 pm; Friday 9:30 am to 2:00 pm.
For travel information for our Wilmslow facility, click here.
Elstree
Waters Ltd.
610 Centennial Park
Centennial Avenue
Elstree
Herts
WD6 3TJ
Download directions
eMail: uk@waters.com
The Waters UK headquarters is located adjacent to M1 junction 4, and is within easy reach of central London and is served by two airports, London Heathrow (28 miles) and London Luton (21 miles).
By Air
- London Heathrow Airport
Flight Information Tel: +44 (0) 870 0000123
- London Luton Airport
Main Switchboard Tel: +44 (0) 1582 405100. A free shuttle bus takes passengers on the 1.5 miles journey from Luton Parkway Station to the terminal.
By Rail
By Taxi
- Borehamwood Radio Cars
Tel: 0208 207 2288
- Jubilee Cars
Tel: 0208 950 8000
Wilmslow, Cheshire
The Corporate Centre for MS Technology Research, Development and Manufacturing
Waters Wilmslow
Stamford Avenue
Altrincham Road
Wilmslow
SK9 4AX
Download directions
eMail: uk@waters.com
Waters Wilmslow is situated minutes away from the most accessible international airport in the UK, gateway to 180 destinations worldwide. Manchester Airport also has regular shuttle feeder flights to London and EU gateways
By Air
By Rail
Accommodation
- We book accommodation on your behalf at a local hotel. Hotel accounts are payable in full on departure.
