Preparative Chromatography Solutions

Preparative Chromatography Solutions

Explore Waters resources for all things liquid chromatography (LC) purification to help your lab with preparative separations workflows.

Explore Waters resources for all things liquid chromatography (LC) purification to help your lab with preparative separations workflows.
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Hear from experts across a wide range of topics, spanning chromatographic methods, laboratory solutions, column chemistry, and software innovations in Chromatography Life, a Waters podcast.
Hear from experts across a wide range of topics, spanning chromatographic methods, laboratory solutions, column chemistry, and software innovations in Chromatography Life, a Waters podcast.

Overview

On-target purification outcomes, every time

Preparative separations are purposeful and practical, meant for isolating targets from mixtures.

Purification outcomes can be off-target, varying by compound purity, recovery and turnaround times. With Waters solutions, including our Optimum Bed Density (OBD) Preparative Columns and Preparative LC Systems, you can ensure more accurate outcomes for your preparative separations. 

Is maximizing target recovery an issue? If peak shape and low sensitivity plague your purification, metal-sensitive compounds may be ‘sticking’ to your stainless-steel column or other metal surfaces in your instrument flow path. Waters MaxPeak High-Performance Surfaces (HPS) Technology can help you achieve predictable scale-up for challenging molecular targets prone to interactions for applications such as organic acids, peptides, and oligonucleotides.

Learn more about preparative solutions for organic acids and peptides

Learn more about preparative solutions for oligonucleotides

Let’s simplify your scale-up.

Let’s simplify your scale-up.

Simple scale-up rules offer practical guidance for purification success on the first try.

Once you’ve learned these five rules, your lab can focus on applying the 5 rules of scale up in LC purification.

Waters OBD Preparative and new Premier OBD Preparative Column Solutions contain robust chromatographic particles designed for the rigors of purification.

With Waters purification solutions, including our highly efficient Preparative Columns, you can directly scale from ultra-performance liquid chromatography (UPLC) and ultra-high performance liquid chromatography (UHPLC) with extended column life and higher recoveries.

We’ll help you crunch the numbers.

We’ll help you crunch the numbers.

Leverage Waters Prep Calculator Tool and Column Coach Tool to help guide you, and support your scale-up from UPLC, UHPLC, or high performance liquid chromatography HPLC to preparative LC.

Take optimum aim with OBD.

Take optimum aim with OBD.

With the Optimum Bed Density (OBD) design of Waters Preparative HPLC Columns, your lab can ensure consistency in column-to-column performance and increase productivity across your workflows.

Waters proprietary and innovative preparative OBD Technology manufacturing procedure combines hardware design, particle characteristics, and exceptional packed bed densities yielding columns with excellent stability, reproducibility, loadability, and high efficiencies. Waters Preparative Column Wall Chart takes the guesswork out of finding the right column for your lab.

“This is one of the routine columns we used for drug discovery isolation of target compounds. It’s been reliable and easy to manage.”
— Lodo Therapeutics

“The SunFire OBD C18 Prep Column made transitioning from my analytical method to a focused-gradient prep scale method fast and easy. Product gave reproducible results which was critical for impurity isolation and identification.” — BioVectra Inc.

“The separation reproduces well from run to run. The separation of small molecules is great with very sharp peaks.” — Warp Drive Bio

“Waters XBridge Oligonucleotide BEH 300 Å C18 5µm OBD columns showed a definitive improvement in removing early impurities.” – Oligo Factory

Solutions

Fully adaptable semi-automated and fully automated systems

Fully adaptable semi-automated and fully automated systems
Preparative HPLC Systems

From semi-automated modular systems to fully automated mass-directed systems, Waters Preparative HPLC Systems and solutions are fully adaptable to meet your lab requirements.

Superior resolution with particles intentionally designed for direct scaling

Superior resolution with particles intentionally designed for direct scaling

Excellent retention and peak shape for target isolations from pH 1-12

Excellent retention and peak shape for target isolations from pH 1-12

Bridged Ethyl Hybrids

XBridge and select Atlantis Preparative Columns utilize Ethylene Bridged Hybrid (BEH) Particle Technology, a silica polymer hybrid packing material. Built to withstand high pH, high temperature and other extreme operating conditions for a wide range of small molecule, biomolecule LC isolations, including peptide, protein and oligonucleotide branded columns which are QC batch tested to ensure consistency.

Waters offers these BEH column chemistries: C18, C8, Shield RP18, Phenyl, HILIC, and Amide in XBridge varieties as well as C18 AX and Z-HILIC as Atlantis options in 2.5, 3.5, 5 and 10 µm particle sizes.

Better peak shape for purification of bases at low pH conditions

Better peak shape for purification of bases at low pH conditions

Charged Surface Hybrids

The benefits of charged surface hybrids materials are significant. With high efficiency and excellent pH stability, Waters CSH Columns are ideal for improved peak shape with bases at low pH. These columns are ideal for improving peak shape for bases when purifying with low pH mobile phase. This includes peptide-branded columns which are QC batch tested to ensure consistency.

Waters offers these column chemistries: C18, Fluoro-Phenyl (PFP), and Phenyl-Hexyl, available in 2.5, 3.5, 5, and 10 µm particle sizes.

Robust silica for direct scaling, to retain polars, or to gain selectivity

Robust silica for direct scaling, to retain polars, or to gain selectivity

High Strength Silica

Waters high strength silica (HSS) technology has excellent low pH stability with high retention and efficiency. This includes peptide-branded columns which are QC batch tested to ensure consistency. With Waters extensive range of column solutions, you can achieve high efficiency for acids, bases, or neutrals, as well as alternative selectivity for polar bases, polar compounds, and aromatic compounds.

Waters offers these HSS Column chemistries: HSS C18, C18 SB, and T3, available in 2.5, 3.5, and 5 μm particle sizes.

Particles designed for maximizing loadability or retention of targets

Particles designed for maximizing loadability or retention of targets

Spherical Silica

Waters SunFire HPLC Columns, available in analytical and preparative dimensions, help you achieve superior mass loading with low pH with state-of-the-art bonded silica designed for excellent low pH stability, superior peak shapes, and high efficiency.

Waters offers these spherical silica chemistries: C18, C8, and Silica in the SunFire variety as well as dC18 and T3 in the Atlantis options in 2.5 (SunFire only), 3.5, 5, and 10 µm particle sizes.

Optimize your instrument productivity

Optimize your instrument productivity
Instrument Services

Waters extensive portfolio of LC and LC-MS instrument support, qualification and services offer quality, knowledge, and reliability for optimum lab productivity.

Make science more accessible with Waters Capital

Make science more accessible with Waters Capital
Payment Solutions

Waters Capital offers a variety of flexible capital, financing, and licensing options, customized for academic institutions.

The data speaks for itself

The data speaks for itself

Predictable scale-up from analytical to prep — we believe prep separations should be fast, and simply, predictable.

Predictable scale-up from analytical to prep — we believe prep separations should be fast, and simply, predictable.
Direct scale-up: UPLC to preparative. 
Direct scale-up: HPLC to preparative. Resolution maintained at increased loads.
Direct scaleup of the sample mixture on XBridge BEH C18 Column.
Improved lifetime and reproducibility matter. Data from a high-throughput drug discovery laboratory: 7,000 injections on an XBridge BEH C18 OBD Prep Column, 130 Å, 5 µm, 19 x 50 mm. Column longevity varies with operating conditions.

Applications

Prep LC is the go-to technique for isolating small molecule targets or impurities from synthetic reaction mixtures, API batches, or other matrices for research.

Prep LC is the go-to technique for isolating small molecule targets or impurities from synthetic reaction mixtures, API batches, or other matrices for research.

Application Notes

Webinars and Resources

Preparative Chromatography Webinar Series

Most Recent Webinars: 5 Rules for Successfully Developing and Scaling Up Your LC Purification and Tips for Getting to Predictable Scaleup in Prep LC

Join us for our On Demand webinar series. Our comprehensive on-demand webinar series is designed to empower you with practical knowledge and expert insights. Whether you’re a seasoned chromatographer or just starting out, these webinars will enhance your expertise and provide actionable tips for immediate application.

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Medical health care laboratory test tubes
  • Brochure

Preparative OBD Columns

Preparative OBD Columns
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  • Brochure

Purification Solutions (Systems)

Purification Solutions (Systems)
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  • Brochure

Applying the 5 Rules of Scale Up in LC Purification

Applying the 5 Rules of Scale Up in LC Purification
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  • Brochure

Waters OBD Preparative Column Ordering Information

Waters OBD Preparative Column Ordering Information
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  • eBook

Purifying Oligonucleotides Guide Book

Purifying Oligonucleotides Guide Book
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  • Primers

Beginner’s Guide to Preparative Liquid Chromatography

Beginner’s Guide to Preparative Liquid Chromatography
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  • Primers

Practical Approaches to Peptide Isolation

Practical Approaches to Peptide Isolation
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  • Tool

Column Coach

Column Coach
Explore Now
  • Tool

Prep Calculator

Prep Calculator
Explore Now
  • Wall Chart

Preparative OBD Columns Wall Chart

Preparative OBD Columns Wall Chart
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  • Wall Chart

Waters Columns, Analytical Standards & Reagents Selection Guide Wall Chart

Waters Columns, Analytical Standards & Reagents Selection Guide Wall Chart
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  • White Paper

Optimum Bed Density (OBD) Columns: Enabling Technology for Laboratory-Scale Isolation and Purification

Optimum Bed Density (OBD) Columns: Enabling Technology for Laboratory-Scale Isolation and Purification
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  • Infographic

Guidance for First-Time Purification Success for Your Precious Targets

Guidance for First-Time Purification Success for Your Precious Targets
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  • Infographic

Preparative Chromatography: Quality by Design

Preparative Chromatography: Quality by Design
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  • Infographic

OBD Preparative Chromatography LC Purification Troubleshooting Guide

OBD Preparative Chromatography LC Purification Troubleshooting Guide
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  • Infographic

OBD Prep Premier LC Purification Infographic

OBD Prep Premier LC Purification Infographic
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  • User Manuals

XBridge Peptide BEH C18, 130Å and 300Å Columns and XBridge Premier Peptide BEH C18, 130Å Columns Care and Use Manual

XBridge Peptide BEH C18, 130Å and 300Å Columns and XBridge Premier Peptide BEH C18, 130Å Columns Care and Use Manual
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  • User Manuals

XSelect Peptide CSH C18, 130Å, 3.5 μm and 5 μm Columns Care and Use Manual

XSelect Peptide CSH C18, 130Å, 3.5 μm and 5 μm Columns Care and Use Manual
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  • User Manuals

XBridge and XBridge Premier Oligonucleotide BEH C18 Columns Care and Use Manual

XBridge and XBridge Premier Oligonucleotide BEH C18 Columns Care and Use Manual
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