As large multinational corporations adopt UPLC Technology and phase out older HPLC systems, there is a transition period where chromatographic methods developed using UPLC Technology must be transferred to and compatible with existing legacy HPLC systems. To make this UPLC Technology adoption strategy successful, one must be able to seamlessly scale between UPLC Technology, HPLC and Preparative LC.  This is only possible if chromatographic column platforms contain HPLC and UPLC particle sizes that exhibit the same selectivities.  The Ethylene Bridged Hybrid (BEH) particle is the particle platform technology behind industry-leading the ACQUITY UPLC BEH and XBridge HPLC column families and is a primary reason behind the success of UPLC Technology.  Waters High Strength Silica (HSS) HPLC columns, available in 3.5 µm and 5.0 µm particle sizes also allow seamless transferability (with 1.8 µm ACQUITY UPLC HSS columns) between UPLC and HPLC system platforms.

ACQUITY UPLC customers who have developed methods with ACQUITY UPLC HSS columns now have HSS HPLC particle sizes and columns that feature:

• 3.5 µm and 5 µm HSS particles packed in the most popular HPLC column formats
  
• The same three chemistries (HSS C₁₈, C₁₈  SB and T3) as ACQUITY UPLC HSS columns
  
• Seamless scalability between HPLC and UPLC Technology