BioSuite Columns

BioSuite Columns

Confidently isolate, analyze, and characterize proteins and peptides

Confidently isolate, analyze, and characterize proteins and peptides

Achieving excellent resolution and recovery of proteins and peptides is critical. Waters BioSuite HPLC Columns for protein and peptide analysis contain high-performance chemistries dedicated to the isolation, analysis, and protein characterization of biomolecules. 

Waters BioSuite HPLC Columns support the array of Waters LC and LC-MS systems and include separation offerings such as ion-exchange, size exclusion, hydrophobic interaction, and reversed-phase columns. With BioSuite Columns for protein and peptide separations, your lab can achieve excellent resolution and recovery of proteins and peptides across different particle and pore sizes, all with the ability to scale from analytical to lab-scale preparative applications.

BioSuite HPLC Columns for protein and peptide separations contain high-performance chemistries dedicated to the isolation, analysis, and characterization of biomolecules. BioSuite Columns
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Specifications

Overview

  • Reversed-phase columns for peptide analysis (silica-based)

  • Reversed-phase columns (polymethacrylate based)  

  • Cation and anion-exchange columns (polymethacrylate based) 

  • Size-exclusion columns (silica-based) 

  • Hydrophilic interaction chromatography (HIC) columns (polymethacrylate based)

Recommended Use: For the separation, lab-scale isolation, and analysis of proteins or peptides.

Features Header

Features Header
BioSuite Size-Exclusion (SEC) Columns
  • Silica-based diol SEC Columns
  • Molecular weight exclusion limits are determined primarily by the selected pore size
  • Available in analytical and semi-preparative column sizes

Explore SEC Columns

BioSuite C18 PA-A Columns
  • Specifically designed for excellent retention of polar peptides
  • Excellent performance in traditional HPLC separations using low TFA concentrations (e.g., 0.025% TFA)
BioSuite C18 PA-B Columns
  • Outstanding performance in separating digests containing hydrophilic, hydrophobic, and basic peptides
  • Superior peak shape and capacity for peptide separations using TFA (e.g., 0.1% TFA)
  • Good choice for separation of large peptide fragments (e.g., Lys-C digests)
BioSuite HIC Columns
  • BioSuite HIC Columns provide a viable separation alternative to reversed-phase chromatography
  • The large 1000 Å pore size accommodates proteins up to 5,000,000 daltons
  • Lab-scale isolation is achievable with the 21.5 × 150 mm BioSuite Phenyl HIC Column
BioSuite Ion-Exchange Columns
  • Four separation chemistries including strong and weak cation (CXC) and anion exchangers (AXC) bonded to a pH stable (i.e., pH 2–12), methacrylic ester-based polymeric resin
  • Provides the flexibility required to develop methods that separate proteins or peptides based upon minor charge differences
  • Speed and superior chromatographic resolution achievable with the Non-Porous (NP) IEX offerings
  • Porous ion exchangers are available for applications requiring greater protein or peptide binding capacity
  • Selected BioSuite Ion-Exchange Columns are offered in PEEK hardware as well as in 21.5 mm I.D. preparative column sizes
BioSuite Phenyl HIC Columns
  • Designed to retain highly polar analytes that are too polar to retain by reversed phase
  • Exceptional chemical stability at high pH and high temperature
  • Ideal for sugar/carbohydrate analysis
  • Exceptional retention of polar analytes spanning a wide range of polarity

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Related

SEC Columns
Analytical size-exclusion chromatography columns are ideal for aggregate, monomer, and fragment analysis. Separate biomolecules by size in solution with Waters SEC Columns.

Peptide Standards and Kits
Develop and validate bioanalytical applications performed on UPLC, HPLC, or LC-MS instrumentation. Waters Peptide, Phosphopeptide, and Protein Digest Standards are high-quality reference reagents.

BioAdvisor
Simplify the process of choosing the best chemistry solutions for your application with the Waters BioAdvisor, which enables you to select an appropriate UPLC/UHPLC or HPLC column and/or chemistry consumable for a desired application, all organized by molecule type.

Offers and Discounts

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BioSuite Columns

Waters BioSuite HPLC Columns provide a powerful, reliable solution for the isolation, analysis, and characterization of proteins and peptides. Designed to deliver superior resolution and recovery, these high-performance columns are ideal for labs requiring consistent, accurate results in biomolecule separations. With a variety of chemistries dedicated to different analytical needs, BioSuite Columns ensure the flexibility and precision needed for modern protein and peptide workflows.

Supporting Waters LC and LC-MS systems, BioSuite Columns offer a comprehensive range of separation techniques, including ion exchange, size exclusion, hydrophobic interaction, and reversed-phase chromatography. These chemistries allow laboratories to achieve optimal separations across different particle and pore sizes, with seamless scalability from analytical to preparative applications. Whether isolating complex peptides or characterizing large proteins, BioSuite Columns provide the tools necessary for success.

Size-Exclusion (SEC) Columns

BioSuite Size-Exclusion (SEC) Columns are silica-based with diol chemistry, delivering exceptional separation of molecules based on size. Available in analytical and semi-preparative sizes, these columns are tailored for accurate molecular weight determinations with pore sizes suited for a variety of exclusion limits. SEC Columns enable precise characterization of biomolecules in both research and industrial settings.

Peptide Separation with BioSuite C18 PA-A and PA-B Columns

For peptide separations, BioSuite C18 PA-A and PA-B Columns are specifically designed to provide excellent retention and resolution. The PA-A Columns excel in traditional HPLC applications with low TFA concentrations, ensuring exceptional retention of polar peptides. In contrast, PA-B Columns are ideal for separating complex peptide mixtures, such as hydrophilic, hydrophobic, and basic peptides, while maintaining superior peak shape and capacity. These columns are particularly well-suited for analyzing large peptide fragments, making them an essential tool for advanced workflows.

Hydrophilic Interaction Chromatography (HIC) Columns

BioSuite Hydrophilic Interaction Chromatography (HIC) Columns offer a unique alternative to reversed-phase chromatography, delivering robust performance for polar analytes. With a 1000 Å pore size capable of accommodating proteins up to 5,000,000 Daltons, these columns provide exceptional chemical stability under high pH and temperature conditions. The 21.5 × 150 mm BioSuite Phenyl HIC Column is specifically designed for lab-scale isolation, supporting both research and preparative workflows.

Ion-Exchange Columns

BioSuite Ion-Exchange Columns provide unmatched flexibility for the separation of proteins and peptides based on minor charge differences. With four chemistries—including strong and weak cation (CXC) and anion exchangers (AXC)—these columns feature a pH-stable methacrylic ester-based polymeric resin. Available in both porous and non-porous options, BioSuite Ion-Exchange Columns meet the needs of diverse applications, whether prioritizing binding capacity or chromatographic resolution. Selected models are also available in PEEK hardware and preparative column sizes, offering additional adaptability for demanding workflows.

Each BioSuite Column is meticulously engineered to deliver consistent, reliable performance, enabling researchers to isolate, analyze, and characterize biomolecules with confidence. By combining advanced chemistries with a range of separation techniques, Waters BioSuite Columns empower laboratories to meet the challenges of modern biomolecule analysis with precision and efficiency.

Experience the difference BioSuite Columns can make in your protein and peptide workflows—achieve superior resolution, recovery, and reliability in every analysis.

FAQs About Waters BioSuite Columns

1. What is the BioSuite column?
The BioSuite column is a high-performance liquid chromatography (HPLC) column designed for the separation and purification of biomolecules.

2. What types of biomolecules can be separated using BioSuite columns?
BioSuite columns are designed to separate a wide range of biomolecules, including proteins, peptides, nucleic acids, and carbohydrates.

3. What is the difference between BioSuite columns and other HPLC columns?
BioSuite columns are specifically designed for the separation and purification of biomolecules and offer superior performance and resolution compared to other HPLC columns.

4. What are the benefits of using BioSuite columns?
BioSuite columns offer high resolution, high recovery, and high purity, making them ideal for a wide range of applications in the life sciences.

5. How do I choose the right BioSuite column for my application?
The choice of BioSuite column will depend on the specific biomolecules you are working with, as well as the desired separation and purification goals. Our technical support team can help you choose the right column for your application.