How to Choose the Right SEC Column for Your Analysis

Maximizing your separation power for challenging analyses such as insulin and monoclonal antibody aggregate analysis is important.

Key considerations:

• Pore size and column dimension is based on the complexity of sample to be separated
• LC System dispersion used for the analysis, as well as sample throughput
• Based on these criteria, the most appropriate UPLC, UHPLC, or HPLC offering can be selected

Learn More

When Speed is Critical for SEC

Harnessing the power of UPLC

ACQUITY UPLC Technology allows scientists the ability to exceed what is obtainable using UHPLC or traditional HPLC separations by improving data quality while increasing sample throughput and productivity.

mAb Aggregate, Monomer, and Fragment Analyses

The BioResolve SEC mAb Guard, Columns, and mAb Size Variant Standard are purposely designed for organizations involved in the research and/or development of monoclonal antibody (mAb) drugs that require accurate quantitation of mAb aggregates (HMWS), monomers, and fragments/clips (LMWS). These offerings provide reliable, high-quality SEC analytical data which addresses internal and regulatory concerns regarding these biotherapeutics. The BioResolve SEC mAb Columns and Guards:

• Provide out-of-the-box performance
• Tested with Waters NIST-based, mAb Size Variant to help ensure consistent column-to-column and batch-to-batch performance, regardless of LC platform 
• "More Than Just a Column."

Adeno-Associated Virus (AAV) Analyses

XBridge BEH SEC 450 Å, 3.5 μm Columns effectively separate and quantitate AAV monomers from their HMW dimers, lower valency multimers, and LMW fragments.

Insulin Aggregate Analysis

Size-exclusion chromatography (SEC) is the USP and EP standard method for the analysis of covalent HMW insulin in therapeutic preparations. Compared to use of traditional HPLC-based SEC methods, significant improvement in insulin component resolution, while reducing analysis time and mobile-phase consumption, can be obtained using a Waters Protein BEH SEC, 125 A, 1.7 μm Column with Waters UltraPerformance LC (UPLC) instrumentation.

BEH SEC Protein Performance Standards

Benchmarking, method development, and troubleshooting

The BEH SEC Protein Standards are specifically designed to help aid in the benchmarking of each set of columns. Each standard contains carefully chosen proteins unique to that chemistry, which has been worked out meticulously over time. These standards are used by Waters to QC the respective material used to produce the UPLC, UHPLC, and HPLC columns. They are also well suited for benchmarking new columns and for monitoring the SEC column performance during repeated use.

Learn More

Easily Transfer Methods without Compromising on Resolution

Flexibility for UHPLC and HPLC

XBridge Protein BEH SEC, 125 Å, 200 Å, and 450 Å Columns containing 2.5 μm (UHPLC) and 3.5 μm (HPLC) particles complement our existing line of UPLC-based SEC offerings for UHPLC or more traditional HPLC-based instrumentation and methods. Easily transfer methods based on laboratory instrumentation, component resolution, or sample throughput needs.