Supercritical Fluid Chromatography (SFC) Columns

SFC Columns

Normal-phase alternative selectivity with reversed-phase ease and reliability

Normal-phase alternative selectivity with reversed-phase ease and reliability

Accessing the power of normal-phase chromatography with the ease and reliability of reversed-phase chromatography is critical for separation scientists like you doing Supercritical Fluid Chromatography (SFC) applications.

With the Torus, Trefoil, and Viridis SFC Column chemistries and Waters SFC instrumentation, now your lab can achieve normal-phase alternative selectivity with reversed-phase ease and reliability - all while ensuring ease of scalability, column-to-column consistency, and reduced method development times. With these high-quality achiral and chiral SFC column chemistries, your lab can address difficult achiral and chiral separations, deliver novel selectivity and robustness, and achieve unequaled speed and unparalleled confidence.


Torus, Trefoil, and Viridis Columns for achiral and chiral SFC Separations. SFC Columns
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Specifications

Overview

  • High-quality, consistent, and reproducible columns
  • Handle achiral and chiral separations with unequaled speed and unparalleled confidence
  • Use for a wider range of compounds in a single sample  
  • Highest efficiency and quality control (QC)-ready robustness
  • Scale from analytical to achiral SFC preparative chromatography
  • Rugged SFC preparative columns available in patented Optimum Bed Density (OBD) technology for excellent column lifetimes

Recommended Use: For use in challenging achiral and chiral separations with unparalleled confidence and unequaled speed.

Torus Column Characteristics

Torus Column Characteristics
Torus 2-PIC (2-Picolylamine) Columns
  • General use achiral column yielding added compound retention and novel selectivity
  • Best choice for applications with both acidic and basic compounds
  • Scalable and available in analytical and preparative column dimensions
  • Enhanced performance compared to conventional 2-EP (2-ethylpyridine)
Torus DEA (Diethylamine) Columns
  • Designed to be orthogonal selectivity compared to the Torus 2-PIC columns
  • Provides superior peak shape for strong bases
  • Delivers complimentary selectivity to the 2-PIC stationary phase
  • Scalable and available in analytical and preparative column dimensions
Torus DIOL Columns
  • Provides more selectivity choices with its high-density diol surface
  • Delivers similar chromatography performance compared to traditional, unbonded silica phases
  • Achieve method robustness when utilized with additives
  • Scalable and available in analytical and preparative column dimensions
Torus 1-AA (1-Aminoanthracene) Columns
  • Superior choice for separating neutral compounds and hydrophobic compounds
  • Provides an orthogonal selectivity to the 2-PIC stationary phase
  • Suitable for achiral SFC method development applications
  • Scalable and available in analytical and preparative column dimensions

Trefoil Column Characteristics

Trefoil Column Characteristics
Trefoil AMY1, CEL1, and CEL2 Columns
  • Provides a broad spectrum of chiral selectivity
  • Polysaccaride-based and designed for chiral compound separations
  • Separate enantiomers, stereoisomers, metabolites, degradants, and impurities with greater resolution and speed
  • Ideal for fast method development applications

Viridis Column Characteristics

Viridis Column Characteristics
Viridis HSS C18 SB Columns
  • Suited for the analysis of glycerides
  • Effective for use in pharmaceutical, food, and chemical applications
  • Scalable and available in analytical and preparative column dimensions
Viridis Silica 2-EP Columns
  • Widely used as high-quality solution for achiral SFC separations
  • Good retention, peak shape, and selectivity
  • Scalable and available in analytical and preparative column dimensions
Viridis Silica Columns
  • Heightened interaction with polar compound functional groups compared to Silica 2-EP
  • Accommodates increased mass loads
  • Good retention, peak shape, and selectivity
  • Available in analytical and preparative column dimensions
Viridis BEH 2-EP (2-Ethylpyridine) Columns
  • Good retention, improved peak shape, and unique selectivity
  • Accommodates increased mass loads
  • Scalable and available in analytical and preparative column dimensions
Viridis BEH Columns
  • Extensive interaction with polar groups such as phospholipids
  • Unique selectivity and improved peak shape for bases
  • Scalable and available in analytical and preparative column dimensions
Viridis CSH Fluoro-Phenyl Columns
  • Good retention of weak bases
  • Provides alternate elution orders for acidic and neutral compounds
  • Scalable and available in analytical and preparative column dimensions

Address the challenges of achiral separations

Simplify SFC method development challenges with Torus Columns. Designed for achiral SFC separations, Torus Columns offer a wide range of selectivity, excellent peak shape, and are suited for method transfer and method scale-up. Torus Columns are offered in both 1.7 and 5 μm chemistries in both analytical and preparative column formats. 

The Torus phases are based on two-stage functionalization of ethylene bridged hybrid (BEH) particles. The initial bonding provides a hydrophilic surface that controls the retention characteristics of the sorbent. This bonding, along with mobile phase additives, minimizes unwanted surface interactions that can lead to retention and selectivity changes over time. The second step of the functionalization is responsible for the individual selectivity and peak shape characteristics of each of the Torus chemistries, resulting in a series of stationary phases with broad ranging selectivities.

Transfer normal-phase methods to SFC chiral methods

Many legacy normal-phase chiral methods have undesirable characteristics, including long run times and the use of chlorinated solvents with tetrahydrofuran (THF) or hexane, which can be expensive to purchase and dispose of. Now, your lab can easily transfer your older SFC methods to the ACQUITY UPC2 System with Trefoil Columns. With simple redevelopment, your lab can obtain new cost-effective methods using inexpensive and non-toxic compressed liquid CO2 as the primary mobile phase. Your lab can then couple those new methods to mass spectrometers to achieve more detailed information.

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Related

ACQUITY UPC2 System
Built upon proven UPLC Technology to enable scientists the ability to address routine and complex separation challenges while delivering reliability, robustness, sensitivity, and throughput never before possible.

QC Reference Materials
Ensure peak LC and LC-MS column and system performance by benchmarking with Waters Quality Control Reference Materials, a portfolio of ready-to-use standards and mixes.

OBD Prep Columns
OBD Technology is the key to predictable preparative column lifetimes. Achieve fast, efficient, lab-scale separations for greater throughput with the ability to scale from UPLC, UHPLC, or HPLC.

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Supercritical Fluid Chromatography (SFC) Columns

Waters Supercritical Fluid Chromatography (SFC) Columns are engineered to meet the demanding requirements of modern separation science, delivering high-quality, consistent, and reproducible results. These columns offer scientists the tools to handle both achiral and chiral separations with unrivaled speed and precision, making them ideal for a wide range of challenging applications. Whether you're working with complex mixtures or separating sensitive compounds, Waters SFC Columns provide the performance, reliability, and scalability your lab needs to succeed.

One of the standout features of Waters SFC Columns is their ability to combine the selectivity of normal-phase chromatography with the ease and reliability of reversed-phase techniques. This unique combination allows you to access normal-phase alternative selectivity while maintaining the simplicity and robustness of reversed-phase chromatography.

Scientists perform well in Supercritical Fluid Chromatography (SFC) applications, where precise, efficient separations are crucial. With Waters SFC Columns, you can achieve the desired separations quickly and with greater confidence, reducing both time and effort in method development.

Waters offers a variety of column chemistries, including the Torus, Trefoil, and Viridis SFC Columns, all designed to provide unmatched speed, accuracy, and reliability for both achiral and chiral separations. These chemistries allow for the separation of a broader range of compounds within a single sample, offering novel selectivity that can address even the most difficult separation challenges. Whether you are working with small molecules, pharmaceuticals, or complex mixtures, Waters SFC Columns provide the flexibility to achieve the results you need, helping you deliver innovative solutions in your research or production processes.

In addition to their superior performance, Waters SFC Columns are also designed for scalability, allowing you to transition from analytical separations to preparative-scale chromatography without sacrificing efficiency or resolution. Waters' patented Optimum Bed Density (OBD) technology, used in their rugged preparative SFC columns, ensures extended column lifetimes, making these columns particularly well-suited for labs that require high throughput and durability. The robust construction of these columns enables them to withstand the most demanding applications, providing the long-term performance your lab depends on for consistent results.

When used in conjunction with Waters' SFC instrumentation, these advanced column chemistries help streamline your workflow by reducing method development times and improving column-to-column consistency. The ease of scalability and the rugged design of these columns ensure that your lab can handle a wide range of SFC applications with minimal downtime or adjustments.

With Waters SFC Columns, your lab is equipped to overcome the most challenging separation tasks, providing the highest level of performance for both routine and complex applications. These columns offer a perfect balance of speed, efficiency, and durability, ensuring that your lab can achieve consistent results with complete confidence.

FAQs about SFC Columns

1. What are SFC Columns?
SFC Columns are a type of chromatography column designed for use with supercritical fluid chromatography (SFC). They are specifically designed to provide high-resolution separations of complex mixtures.

2. What are the benefits of using SFC Columns?
SFC Columns offer a number of benefits, including high resolution separations, low backpressure, and low solvent consumption. They are also highly efficient and can be used for a wide range of applications.

3. What types of SFC Columns are available?
Waters offers a range of SFC Columns, including columns for normal-phase, reversed-phase, and chiral separations.

4. How do I choose the right SFC Column for my application?
The best SFC Column for your application will depend on the type of separation you need to perform. Waters offers a range of columns for different types of separations, so you should be able to find the right column for your needs.

5. Are SFC Columns compatible with other chromatography systems?
Yes, SFC Columns are compatible with other chromatography systems, including HPLC and UHPLC.