SONAR
Stop looking, start seeing
Waters has more than two decades of close involvement in introducing, developing, and commercializing modes of both data dependant and data independent acquisition (DIA), culminating in the SONAR and HDMSE technologies deployed across the MS portfolio.
The strength of SONAR is its increased selectivity with rapid scan rates, which translates to cleaner MS/MS data and allows both discovery and quantification from a single injection using commonly accepted and established workflows. With SONAR, your lab can confidently achieve the right result the first time, giving you improved data quality through high selectivity and more reliable database searches.
Overview
- All the information required for quantification and identification in a single injection
- Scan rates compatible with UPLC/UHPLC speeds for reliable quantitation
- More reliable database searches due to cleaner MS/MS spectra
- More efficient lab workflows by removing time-consuming method development associated with MS/MS analysis
How will SONAR improve my results?
With SONAR, you can get the routine MS/MS data your lab needs without time-consuming method development or the risk of missing information with data-dependent options. The main difference between DIA and SONAR is the behavior of the quadrupole. Instead of remaining open and transmitting all ions, it slides over the selected mass range, increasing selectivity while still operating at UPLC/UHPLC speeds. Each scan is made up of 200 spectra, and filtering of the precursor ions by the quadrupole increases the method selectivity.
The resolving quadrupole creates an extra dimension to the data. Compounds that are chromatographically co-eluting are now separated and recorded individually, and library searches are more reliable. SONAR provides data for quantitation and identification in a single injection.
How does SONAR work?
There are three instrument parameters that are key to SONAR:
- With SONAR, the quadrupole is active. The resolving quad slides over a selected mass range during each MS scan.
- From scan to scan, the collision cell is alternating between high, and low collision energy. This data is collected in two data channels that the software aligns to associate the precursor and fragment ions.
- Each scan contains 200 spectra. The three dimensional data allows the MS/MS data to be reported separately.
This diagram demonstrates how the quadrupole slides over the mass range of interest, transmitting ions in sequence. Precursor and fragment ion data is recorded, generating MS/MS results that can be viewed and processed individually. The resulting cleaner data is easier to interpret and increases the reliability of library searches.
Increase confidence in results
With SONAR, your lab can confidently achieve the right result the first time, giving you improved data quality through high selectivity and more reliable library searches. SONAR also saves time in the lab, delivering improved efficiency through:
Routine MS/MS without method development
Cleaner spectra allowing automated data interpretation
Quantification and identification from a single injection
Operation at UPLC/UHPLC speeds
Resources
Documents
- Scanning Quadrupole Data-Independent Acquisition, Part B: Application to the Analysis of the Calcineurin-Interacting Proteins during Treatment of Aspergillus fumigatus with Azole and Echinocandin Antifungal Drugs
- Scanning Quadrupole Data-Independent Acquisition, Part A: Qualitative and Quantitative Characterization.
- Scanning Quadrupole Data-Independent Acquisition, Part B: Application to the Analysis of the Calcineurin-Interacting Proteins during Treatment of Aspergillus fumigatus with Azole and Echinocandin Antifungal Drugs
- Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning quadrupole data-independent acquisition strategy
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