ACQUITY APC Columns

ACQUITY APC Columns

Achieve superior speed and resolution for polymer characterization

Achieve superior speed and resolution for polymer characterization

Achieving superior performance and reliability with high resolution polymer characterization can be a challenge. ACQUITY Advanced Polymer Chromatography (APC) Columns are designed for polymer scientists like you, with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods. With these columns, you can quantify and characterize polymer samples with confidence and accuracy while maximizing productivity.

The Ethylene Bridged Hybrid (BEH) particles used in ACQUITY APC Columns enable rapid solvent switching and the ability to use multiple conditions for the same bank of columns. Conventional polymeric stationary phases are dedicated to a single set of analytical conditions and solvent because the chromatographic bed is vulnerable to shrinking and swelling. By eliminating this undesirable behavior, ACQUITY APC Columns allow for maximum robustness and method development flexibility.

ACQUITY APC Columns for separating complex synthetic polymer and macromolecular species. ACQUITY APC Columns
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Specifications

Overview

  • Eliminate the shrinking and swelling that often occurs with polymeric stationary phases upon solvent switching with a stable chromatographic column bed 
  • Achieve unmatched separation speed, resolution, and chromatographic efficiency from optimized pore volumes and particle size
  • Meet workflow requirements with 1.7 and 2.5 μm columns for organic and aqueous size-based separation
  • Achieve industry-leading column-to-column reproducibility with exceptionally reliable columns from a tightly controlled column manufacturing process

Recommended Use: For rapid and accurate chromatographic characterization of synthetic polymer and macromolecular species. 

ACQUITY APC Column Featured Technologies

ACQUITY APC Column Featured Technologies
BEH Technology
  • Rugged hybrid organic/inorganic base particle
  • Improved peak shape for bases- low silanol activity
  • Wider operating pH range for method development flexibility
  • Mechanical strength for maximum column efficiency at UHPLC operating pressures

ACQUITY APC Column Characteristics

ACQUITY APC Column Characteristics
ACQUITY APC XT Columns
  • Designed to provide high efficiency sized-based separations for organic soluble polymer characterization
  • Uses BEH particle technology
  • Stable column bed that resists shrinking and swelling for easy mobile-phase solvent switching
  • Features an extended temperature range up to 90 °C

Designed for organic solvent-based separations

ACQUITY APC AQ Columns
  • Designed to provide high efficiency sized-based separations for water soluble polymer characterization
  • Uses BEH particle technology
  • Robust separations up to 45 °C

Designed for aqueous-soluble polymer separations

Unprecedented capacity for rapid solvent switching

Use multiple conditions for the same column bank with rapid solvent switching using the rigid hybrid particles in ACQUITY APC Chromatography Columns. Conventional polymeric phases are often dedicated to single analysis conditions because the chromatographic bed is vulnerable to shrinking and swelling that can be potentially harmful to the column. By eliminating this constraint, ACQUITY APC Chromatography Columns provide more freedom for method development and deliver advanced polymer characterization for a diverse range of samples.

Reduce calibration time by a factor of 3-5x and increase resolution

With long conventional Gel Permeation Chromatography (GPC) run times of up to one hour or more, analyses of standards and samples can be quite time-consuming. In addition to long GPC run times, the large extra-column volume of conventional GPC systems can result in peak bandspreading, reducing the resolution and thus accuracy of the calibration points. Combined with the lower dispersion ACQUITY APC System, small particle ACQUITY APC Columns deliver less bandspreading and narrower, sharper peaks compared to the conventional GPC system. Additionally, robust sub-3-µm APC Column Technology supports higher flow rate and backpressures, thereby improving resolution.

Ensure reproducible column performance

Reproducible column performance is one of the most critical factors for achieving long-term method reliability during polymer characterization. Each step of our column manufacturing process is monitored and tightly controlled to maintain unmatched product reproducibility. Every test, from raw material characterization to final batch analysis, is designed to ensure that ACQUITY APC Columns perform the same from column-to-column, batch-to-batch, and year-to-year.

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Related

ACQUITY Advanced Polymer Chromatography Calibration Standards Kits
Achieve better data with ACQUITY APC Calibration Standards & Kits, specifically designed to match the molecular weight range of the ACQUITY APC columns. Available in polystyrene and polymethylmethacrylate configured into Low, Middle, and High Molecular Weight Calibration Kits.

ACQUITY Advanced Polymer Chromatography System
Breakthrough technology for high-resolution characterization of polymers. Delivers more information about your polymers faster than ever before.

APC Column Selector
Simplify the process of choosing the best APC Column and calibration standards for your polymer characterization, with the APC Column Selector, an interactive column selection tool.

Offers and Discounts

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ACQUITY APC Columns

At Waters, we know that achieving superior performance and reliability in high-resolution polymer characterization can be a significant challenge. Our ACQUITY Advanced Polymer Chromatography (APC) Columns are designed specifically for polymer scientists, offering novel capabilities that surpass conventional Gel Permeation Chromatography (GPC) methods in chromatographic efficiency, speed, and resolution. With these advanced columns, you can quantify and characterize polymer samples with confidence and accuracy while maximizing productivity.

The Ethylene Bridged Hybrid (BEH) particles used in ACQUITY APC Columns are the heart of the technology, enabling rapid solvent switching and the use of multiple conditions for the same set of columns. Conventional polymeric stationary phases are often restricted to a single set of analytical conditions and solvents due to the vulnerability of the chromatographic bed to shrinking and swelling. By eliminating this undesirable behavior, ACQUITY APC Columns provide maximum robustness and flexibility in method development, ensuring reliable and reproducible results across diverse analytical conditions.

ACQUITY APC Columns offer unmatched separation speed, resolution, and chromatographic efficiency thanks to optimized pore volumes and particle sizes. Available in 1.7 and 2.5 μm sizes for both organic and aqueous size-based separations, these columns meet a wide range of workflow requirements. The rigid hybrid particles allow for unprecedented rapid solvent switching, allowing multiple conditions to be used with the same column bank, which enhances method development flexibility and delivers advanced polymer characterization.

One of the significant advantages of ACQUITY APC Columns is the ability to reduce calibration time by a factor of 3-5x while increasing resolution. Conventional GPC systems can have long run times of up to an hour or more, and the large extra-column volume can lead to peak band spreading, reducing resolution and accuracy. In contrast, the ACQUITY APC System, combined with small particle ACQUITY APC Columns, delivers less band spreading and narrower, sharper peaks. This results in higher flow rates and back pressures, thereby improving resolution and reducing overall analysis time.

Reproducibility is a critical factor in achieving long-term method reliability for polymer characterization. Each step of the ACQUITY APC Column manufacturing process is meticulously monitored and tightly controlled to ensure unmatched product reproducibility. From raw material characterization to final batch analysis, every test is designed to guarantee consistent performance from column to column, batch to batch, and year to year.

Waters' ACQUITY APC Columns provide an advanced solution for the rapid and accurate chromatographic characterization of synthetic polymers and macromolecular species. By eliminating the limitations of conventional polymeric stationary phases, these columns offer superior speed, resolution, and chromatographic efficiency. Their robust design and flexible solvent-switching capabilities make them an invaluable tool for any polymer scientist looking to enhance their analytical capabilities and productivity.

With ACQUITY APC Columns, you can achieve industry-leading performance and reliability, ensuring your polymer characterization methods are both efficient and accurate.

FAQs About Waters ACQUITY APC Columns

1. What is the composition of Waters ACQUITY APC Columns?
Waters ACQUITY APC Columns are made of silica-based particles with a proprietary bonding technology that provides high efficiency and selectivity for reversed-phase chromatography.

2. What is the recommended pH range for using Waters ACQUITY APC Columns?
Waters ACQUITY APC Columns are recommended for use in the pH range of 2-8.

3. What is the maximum operating pressure for Waters ACQUITY APC Columns?
Waters ACQUITY APC Columns have a maximum operating pressure of 15,000 psi.

4. What is the recommended temperature range for using Waters ACQUITY APC Columns?
Waters ACQUITY APC Columns are recommended for use at temperatures between 30-60°C.

5. What applications are Waters ACQUITY APC Columns suitable for?
Waters ACQUITY APC Columns are suitable for a wide range of applications, including pharmaceuticals, biopharmaceuticals, food and beverage, environmental, and clinical research.