In this technical brief, the BioResolve RP mAb Polyphenyl Column is shown to be able to facilitate improved mAb subunit separations. The column is shown to be used with conventional RPLC-MS methodologies to improve the capabilities of subunit-level analysis. With enhanced chromatographic resolution and through careful optimization of mass loads, it has been possible to better clarify sample heterogeneity providing more insight into mAb structure and function.
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