Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
Dwell Volume and Extra-Column Volume: What Are They and How Do They Impact Method Transfer
Method transfer or method scaling across different LC instrument platforms can be affected by both dwell volume and extra-column dispersion. Each characteristic will have a different effect on the chromatographic separation.
- Dwell volume impacts the retention times of a gradient separation, but can also affect selectivity, particularly for early eluting compounds.
- Extra-column dispersion or volume impacts peak width, resolution, and the overall efficiency of a separation.
In this review, the differences of these attributes across of variety of HPLC, UHPLC, and UPLC instruments will be examined, specifically for their impact on transferring a method. We will review strategies to minimize that impact. These recommendations will follow the USP <621> guidelines.
| Title | Format | File Size |
|---|---|---|
| Download (720005723en) | 1007.76kB |
