Assessing Repeatability of a Peptide Mapping Method Using the ACQUITY Arc Bio System

Library Number:
APNT134971523
Part Number:
720006224EN
Author(s):
Brooke M. Koshel, Robert E. Birdsall, and Ying Qing Yu
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Technical Notes
Related Products:
 
 
 
 

In this study, a trypsin digest of trastuzumab was used to demonstrate repeatability of a peptide mapping using the ACQUITY Arc Bio System.

The retention time percent RSD for 102 peptides ranged from 0.025–0.05% for the majority of peptides. The percent RSD for the area of eight peptides selected throughout the gradient ranged from 0.26–0.93%. The percent RSD for both metrics demonstrated excellent instrument precision, which is important for generating reliable data and providing confidence in results.


Title Format File Size
Download PDF PDF 565.62kB

Back To Top Back To Top