This application note shows how the availability of the HSS Cyano phase in 5-, 3.5-, 2.5-, and 1.7-μm particle sizes facilitates scaling between different instrument platforms (HPLC and UPLC ). Method conditions can be easily scaled for different column configurations using the ACQUITY UPLC Columns Calculator. Scaling methods to utilize the XSelect HSS Cyano XP 2.5 μm Columns maximizes productivity with existing HPLC instrumentation. Additional method transfer to UPLC conditions offers the greatest savings in time and resources.
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