Electron transfer dissociation (ETD) is a powerful fragmentation technique for determining modification sites of labile post-translational modifications (PTMs), which are often difficult to characterize using collision induced dissociation (CID). This application note offers details on glycopeptide characterization using ETD fragmentation.
ETD fragmentation enhanced by a "super charging" reagent improves the overall glycopeptide fragmentation efficiency. The SYNAPT G2-S HDMS System equipped with an ETD source proves to be an indispensible tool for protein glycosylation analysis.
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