Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
Improving Resolution and Column Loading Systematically in Preparative Liquid Chromatography for Isolating a Minor Component from Peppermint Extract
This application note illustrated a systematic preparative HPLC method development to isolate a minor component from peppermint extract using an AutoPurification System. The overall workflow included screening different column chemistries, applying focused gradients, scaling up, and employing an ACD injection scheme. With proper scale-up from an optimized analytical chromatographic condition, employing ACD increased the sample loading by five-fold while maintaining the resolution on the preparative scale. The techniques demonstrated in this case study have general applicability for laboratories routinely performing natural product isolation using preparative HPLC.
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