Using Isocratic Preparative Liquid Chromatography as a Quick First-Step Purification for Natural Product Isolation
In this application note, a workflow to systematically develop isocratic prep LC methods for target compound isolation from complex natural product mixtures is demonstrated. The workflow starts with a generic LC analysis from which the elution %B of the target compound is calculated. The target compound is then isolated from the sample using an isocratic preparative LC method run at the calculated %B, leading to the collection of target compound with purities ranging from 73% to 100%. The isocratic prep LC methods were also proven rugged and reproducible. Compared to the commonly used gradient methods, the isocratic prep chromatography dramatically reduced the total run time and solvent usage, resulting in improved overall productivity and cost-effectiveness of the purification process. The proposed workflow is ideally suited as a quick, first step in a multi-step process for target compound isolation from natural products.
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