Forced Degradation Analysis of Omeprazole Using CORTECS 2.7 μm Columns
A forced degradation study of Omeprazole was performed using a CORTECS C18+, 2.7 μm, 4.6 x 150 mm Column on an Alliance HPLC System equipped with both a PDA and QDa Detector. This column exhibited high resolution as shown for two critical pairs of isobaric degradants, as well as backpressures compatible with traditional HPLC (<5,000 psi). The use of the CORTECS C18+ stationary phase gave sharp and symmetrical peak shape for Omeprazole (a weak base) and its degradants, even when using low pH, low ionic strength pH modifiers (e.g. formic acid). The combination of CORTECS 2.7 μm Columns, as well as PDA and mass detection using the QDa Detector allows for the rapid separation and identification of closely eluting compounds in complex sample mixtures. On an HPLC system, 150 mm length CORTECS 2.7 μm Columns can be used at an appropriate flow rate without exceeding the pressure limits, thus maximizing the potential for increased resolution in HPLC.
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