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Polar Basic Drugs in Environmental Samples; Improved Analysis Using a New High Efficiency UPLC Column for HILIC
The purpose of this application note is to demonstrate the CORTECS UPLC HILIC Column for determination of highly polar basic compounds. In this application, a number of these compounds are determined in a spiked river water sample. Sample preparation was performed using the EPA 1694 method modified for use with a smaller sample volume (100 mL rather than 1000 mL) and a smaller Oasis HLB Cartridge (150 mg compared with 1000 mg). The same enrichment factor was maintained and similar performance was observed compared with the protocol given in method 1694.1 Sample preparation time was reduced from about 4 hours to about 1 hour.
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