Transfer of an SEC Method for Monoclonal Antibody Analysis from HPLC to UHPLC Using the ACQUITY Arc System
The ACQUITY Arc System is an LC platform designed to bridge the gap between HPLC and UPLC, allowing users to seamlessly transfer methods across laboratories.
The introduction of the ACQUITY Arc System recognizes the need to emulate legacy HPLC techniques, but also offers the advantage of adopting UHPLC technology if desired. An SEC method was successfully transferred from an Agilent 1100 Series HPLC to the ACQUITY Arc System without changing any method parameters. The ACQUITY Arc System demonstrated a high degree of reproducibility, which is important when verifying product consistency. Finally, the transition from SEC-HPLC to SEC-UHPLC showed improved resolution and a shorter run time while maintaining comparable peak area percentages to those obtained under HPLC conditions.
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