Transferring Methods for Monoclonal Antibody Analysis from an Agilent 1260 Infinity Quaternary LC System to an ACQUITY Arc System

Library Number:
APNT134908703
Part Number:
720005787EN
Author(s):
Brooke M. Koshel, Robert E. Birdsall, and Ying Qing Yu
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
 

This application note demonstrates method equivalency between an Agilent 1260 Infinity Quaternary LC System and an ACQUITY Arc System for transfer of isocratic and gradient methods for monoclonal antibody analysis.

The ACQUITY Arc System combined with Gradient SmartStart Technology minimizes any differences in dwell volume between two systems, resulting in comparable relative retention times. These results demonstrate the ability of the ACQUITY Arc System for emulating HPLC/UHPLC methods, and its ability to streamline the method transfer process.


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