Many countries have maximum permitted levels for mycotoxins that pose the highest risk in different foodstuffs. Multi mycotoxins methods based upon LC-MS/MS offer an effective and efficient option to determine all regulated mycotoxins in a single method. Here we demonstrate the performance of a simple and quick approach to sample preparation in combination with LC-MS/MS, with no clean-up. The excellent sensitivity offered by the Xevo TQ-XS tandem quadrupole mass spectrometer was selected to ensure that performance can easily meet regulatory limits despite using a simple dilute and shoot approach. The use of 13C-labelled analogues as internal standards was investigated and compared to the conventional external standard method. Carryover is one of the main issues when dealing with some mycotoxins (e.g. fumonisins) but this can be minimized by the careful selection of injector, wash cycle, wash solvents and chromatographic conditions. The performance of the method was initially evaluated in wheat and then further verified by analysis of other cereal commodities: wheat, oats, maize, rice, and buckwheat-based food products, according to criteria described in European regulations and SANTE guidelines. Subsequently, the performance of the method was verified by an interlaboratory study. The incorporation of 13C-labelled internal standards within the analytical workflow leads to enhanced method performance and are therefore recommended as an efficient approach to correct for both matrix effects, and the inevitable analyte losses during the generic sample preparation.