The area of anionic polar pesticide analysis has been evolving over the past 10 years with the adoption of generic extraction methods, such as the QuPPe¹ method, enabling laboratories to take a multi-residue approach for the analysis of these challenging analytes. The extraction performance of the QuPPe method is well documented and is a common approach for the extraction of polar pesticides from various food matrices. With the recent developments in MS detector technology, lower limits of detection and quantification can be achieved for this analysis by utilizing the enhanced negative ion sensitivity of the Xevo™ TQ Absolute Mass Spectrometer. This poster presents results from the performance of the LC-MS/MS method where limits of quantification of 0.5 µg/kg in cucumber and 2 µg/kg in cereal samples are achievable. The trueness of the LC-MS/MS method was assessed over 10 injections at 1 and 10 µg/kg in cucumber matrix standards and at 10 and 50 µg/kg wheat flour matrix standards. Trueness in cucumber was between 91 to 117% with RSDs between 0.6 to 8.7% and between 96 to 104% in wheat flour with RSDs between 0.5 to 9.2%.