Fast and Robust LC-UV-MS Based Peptide Mapping Using RapiZyme™ Trypsin and IonHance™ DFA
Peptide mapping workflows are typically complex and time-consuming. Hence, there is a need for simple, reproducible, and robust workflows that can facilitate reliable QC analyses and monitoring, as in the case of the biopharmaceutical industry. Sample preparation is a crucial step in these workflows and is usually time-consuming; however, RapiZyme Trypsin can enable users to reduce their sample preparation times, as demonstrated in this application note. Furthermore, the use of IonHance DFA as a mobile phase additive allows a robust LC-UV-MS peptide mapping experience through high-quality and reproducible data acquisition. This type of method show promise for becoming a platform method and enabling the timely assessment of protein therapeutical products in highly regulated environments.
