Development and Optimization of a HILIC-MS Separation of 17 Free Amino Acids using an XBridge Premier BEH Amide Column

Library Number:
APNT135122837
Part Number:
720008010EN
Author(s):
Kenneth D. Berthelette, Jamie Kalwood, Kim Haynes
Source:
Waters
Content Type:
Application Notes
Content Subtype:
Application Notes
Related Products:
 
 
 
 

In this application note, we describe the development of a HILIC separation of seventeen free amino acids using a stepwise, methodical approach for column and mobile phase pH screening and method optimization. Due to the lack of significant chromophores, mass detection was employed for these analytes, using an ACQUITY QDa Detector.

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