Scaling Reverse-Phase and Hydrophilic Interaction Liquid Chromatography (HILIC) Methods to HPLC

Bibliotheks-Nummer:
PSTR135136320
Autor(en):
Margaret Maziarz, Paul Rainville, Stephanie Harden
Quelle:
Waters
Inhalt:
Poster
Untergruppe:
HPLC
Verwandte Produkte:
 
 
 
 
 

This poster describes the process of scaling chromatographic methods between different liquid chromatography (LC) instruments. It emphasizes maintaining the ratio of column length to particle size to preserve separation quality. The study involved scaling methods developed on 1.7 µm particle size columns to larger particle sizes for HPLC analysis. Adjustments were made for system dwell volumes to ensure consistent chromatographic separation. The scaled methods achieved equivalent separation and assay results as the original methods, demonstrating successful method migration across different LC systems.


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