Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
Scaling Reverse-Phase and Hydrophilic Interaction Liquid Chromatography (HILIC) Methods to HPLC
This poster describes the process of scaling chromatographic methods between different liquid chromatography (LC) instruments. It emphasizes maintaining the ratio of column length to particle size to preserve separation quality. The study involved scaling methods developed on 1.7 µm particle size columns to larger particle sizes for HPLC analysis. Adjustments were made for system dwell volumes to ensure consistent chromatographic separation. The scaled methods achieved equivalent separation and assay results as the original methods, demonstrating successful method migration across different LC systems.
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