Improving Chromatographic Resolution of the JECFA Method for the Analysis of Steviol Glycosides
This poster describe about Steviol glycosides (SG) are often used as non-caloric sweeteners in foods and beverages. There are more than 40 SG have been identified. The most abundant ones are rebaudioside A (Reb A) and stevioside (SV). However, some minor SG are becoming more readily available and are in demand due to their higher sweetness intensity and less bitter aftertaste. The Food and Agriculture Organization of the United Nations, and the World Health Organization (FAO/WHO) Joint Expert Committee on Food Additives (JECFA) has recently published a SG monograph, in which a reversed phase LC was recommended for the determination of the major and the minor SG by LC-UV and LC-UV-MS, respectively. However, the chromatographic resolution is not adequate enough.
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