Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
4.6 mm |
Length |
100 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH Amide Column, 130Å, 3.5 µm, 4.6 mm X 100 mm, 1/pk
Add the Waters XBridge BEH Amide Column to your lab in order to be able to retain extremely polar compounds, including carbohydrates and sugars. Easily conduct the separation of polar analytes across a variety of features such as polarity, structural moiety, and pKa by using the XBridge Amide analytical column, which provides you with a chemically stable, trifunctionally bonded amide phase that is stable across a wide pH range of 2-11.
In order to offer unprecedented stability and versatility in HILIC separations lab equipment, the XBridge BEH Amide Column is based on Waters’ novel ethylene bridged hybrid (BEH) particle and makes use of a chemically stable, trifunctionally bonded amide phase. Waters has developed this ability as an innovative approach to overcome the challenge of retaining and separating extremely polar compounds in HILIC stationary phases. The XBridge BEH Amide Column has the same selectivity as ACQUITY UPLC BEH Amide columns, which makes it possible for scientists to easily transfer separations seamlessly between HPLC and UPLC technology platforms.
Since it has been designed specifically to retain polar analytes and metabolites that are too polar to retain using reversed-phase chromatography, the lab equipment is easily able to support the use of a wide range of mobile phase pH. This particular listing is ideally suited for the analysis of carbohydrates with the 3.5 µm particle size and 130Å pore size. Using the XBridge BEH Amide Column enables you to attain high resolution and high-speed analysis in complex sample matrices while maintaining or improving the chromatographic resolution.
The XBridge BEH Amide Column is available in different variations to suit your different analysis needs. You can easily shop for lab equipment such as the variants of the column and other products that work in conjunction with them directly from our online store.
Waters recommends that you purchase the XBridge BEH Amide VanGuard Cartridge, 130Å, 3.5 µm, 3.9 mm X 5 mm, 3/pk along with the XBridge analytical column in order to extend the lifetime of the column by removing harmful contaminants.
What Pressure Works With XBridge BEH Amide Column?
The XBridge BEH Amide Column can tolerate up to 6,000 psi (400 Bar or 40 Mpa), although it is recommended that the pressure range be kept between 4,000-5,000 psi so that the column continues to deliver optimum results.