Waters has a solution for your peptide separations.
We offer a wide variety of reversed-phase columns for the analysis and lab-scale isolation of a wide range of peptides (large and small, acidic and basic, hydrophobic and hydrophilic). These unique columns are based on innovative particle technologies that include Waters' Ethylene-Bridged Hybrid (BEH) and Charged Surface Hybrid (CSH™) offerings which are exclusively quality tested with tryptic protein digests to help ensure consistent batch-to-batch and column-to-column performance consistency. Nano, capillary, analytical, and lab-scale preparative columns are available in a wide range of particle sizes (1.7 µm to 10 µm) that address specific peptide application needs. In addition to these premier hybrid-based particle offerings, Waters also offers a series of silica-based, reversed-phase column chemistries that provide different peptide separation selectivities.
Select the right LC column using the following criteria.
BASE Particle Type
Particle TECHNOLOGY
Particle SIZE
Base Particle Type
SILICA PARTICLES
HYBRID PARTICLES
Waters Peptide CSH C18 Columns expand upon Waters BEH Technology™ in a unique way. The addition of a well-controlled, low level surface charge makes this particle particularly well suited for LC-MS applications using non-TFA mobile phases.
Waters Peptide HSS Columns are designed for separations where silica-based selectivities are desired or if separations are insufficient when using the BEH and CSH columns. This stationary phase provides increased retentivity for peptides.
Waters Peptide BEH C18 Columns provide the most flexibility for the development of UPLC, UHPLC, HPLC, and lab-scale peptide applications ranging from synthetic peptides to complex peptide maps.
Particle Technology
BEH (Ethylene-Bridged Hybrid)
CSH (Charged Surface Hybrid)
HSS (High Strength Silica)
- Ideally suited for separation of a wide range of peptides: large and small, acidic and basic, hydrophilic and hydrophobic
- Stable across a wide pH range (pH 1-11) to change selectivities by adjusting pH
- High temperature stability (up to 80 °C) expands method development capabilities
- Outstanding peak capacity and superior peak shape in trifluoroacetic acid (TFA) or formic acid (FA) ion pair eluents when compared to 100% silica based C18 columns
- Two pore sizes (130Å and 300Å) provide different separation selectivities for a wide range of peptides and small proteins
Trifunctional C18 ligand, fully end-capped, bonded to Ethylene-Bridged Hybrid (BEH) substrate.
- Outstanding peak capacities with formic acid for MS-based applications
- Excellent performance with TFA for optical based applications
- Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities
- Offers unique selectivity when compared to BEH C18 columns
- Optimal for separations from pH 1-5
- The 130Å pore size is best suited for compounds less than 10,000 Daltons
- Separations that require silica-based selectivity
- Separations that necessitate increased peptide retention
- Ideally suited for small, hydrophilic peptides
Trifunctional C18 ligand, fully end-capped, bonded to Charged Surface Hybrid (CSH) substrate.
Trifunctional C18 ligand, fully end-capped, bonded to High Strength Silica (HSS) substrate.
Particle Size
Particle Sizes (scalable)
1.7 µm, 3.5 µm, 5 µm , 10 µm
1.7 µm, 2.5 µm, 3.5 µm, 5 µm
1.8 µm, 2.5 µm, 5 µm
Pore Diameter
130Å, 300Å
130Å
100Å
Column Quality Control
Preparative Columns
XBridge Peptide BEH C18 OBD
Prep Columns
XSelect Peptide CSH C18 OBD
Prep Columns
XSelect HSS T3 10 mm Columns
Method Validation Kits
Available
Available
Not available
Columns specifically QC tested with tryptic digests of cytochrome c (P/N 186006371) to ensure column-column consistency with validated methods.
Columns specifically QC tested with tryptic digests of cytochrome c (P/N 186006371) using 0.1% FA-containing eluents for LC-MS applications.
Unlike the hybrid particles, the HSS particles are not specifically QC tested using tryptic digests, but they are still manufactured with strict, quality control tests for batch-to-batch consistency.
ACQUITY UPLC Peptide BEH
C18 Columns
ACQUITY UPLC Peptide CSH
C18 Columns
ACQUITY UPLC HSS T3 Column
UPLC Columns
HPLC/UHPLC Columns
Peptide Columns Debates
A video series of debates from our leading column candidates for reversed-phase peptide separations to help you choose the right columns for your peptide analyses.
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Peptide Standards and Kits
For the development, validation, and troubleshooting of peptide applications.
More info
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