Symmetry HPLC Columns

Symmetry HPLC Columns

Silica-based columns that exceed HPLC column performance expectations

Silica-based columns that exceed HPLC column performance expectations

Reproducible HPLC columns are essential when developing analytical methods for pharmaceutical and biopharmaceutical products. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.

Available in column and guard formats, Symmetry Columns are manufactured using high purity silica and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.

Symmetry HPLC Column Family Symmetry HPLC Columns
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Specifications

Overview

  • Deliver maximum reproducibility batch-to-batch and column-to-column
  • Provide superior peak shape
  • Achieve high recoveries of peptides and proteins
  • Manufactured using high purity silica and tightly controlled manufacturing processes

Recommended Use: For rugged and reproducible HPLC column performance.

Symmetry Column Characteristics

Symmetry Column Characteristics
Symmetry C18 and C8
  • General purpose
  • High efficiency
  • Reversed-phase columns

Delivers maximum reproducibility

Symmetry Shield RP18 and RP8
  • General purpose
  • High efficiency
  • Reversed-phase columns

Superior peak shape for basic analytes at neutral pH

Symmetry300 C18 and C4
  • Quality control tested with peptides
  • Wide pore
  • Reversed-phase columns

Provides high recoveries of peptides and proteins

Rugged and reproducible HPLC column performance

With Symmetry HPLC Columns, you’ll achieve reliable column-to-column and batch-to-batch reproducibility. Symmetry’s narrow column specification ranges minimize variation, giving you the confidence that the methods you use today will continue to be used in the future.

Superior peak shape

Symmetry Shield Columns feature the Waters patented embedded polar group technology that shields the silica's residual silanols from highly basic analytes and improves overall peak shape. Placing the embedded polar group close to the silica surface further reduces surface silanol activity, and the embedded polar group technology allows you to improve chromatographic peak shape and selectivity in the most challenging of method conditions.

Deliver high peptide recovery

All Symmetry300 columns are QC tested with peptide samples to ensure exceptional batch-to-batch reproducibility. These wide-pore (300 Å), reversed-phase chromatography columns deliver superior performance when simultaneously separating hydrophilic and hydrophobic peptides, neutral, and highly basic peptides. With a Symmetry300 column, you can expect better performance with either high or low mass loads.

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Symmetry HPLC Columns

Symmetry HPLC Columns are designed to exceed the performance expectations of traditional silica-based HPLC columns, offering unmatched reproducibility and reliability. For pharmaceutical and biopharmaceutical applications where consistency in analytical methods is crucial, Symmetry HPLC Columns deliver superior column-to-column and batch-to-batch reproducibility, helping streamline the analytical process and maintain quality control.

Manufactured with high-purity silica, Symmetry Columns provide reproducible and rugged HPLC performance. These columns are available in both column and guard formats, making them versatile and adaptable to a wide range of applications. Symmetry's narrow column specification ranges are designed to minimize variation, ensuring that analytical methods remain reliable over time. Whether you're working on early-stage development or long-term batch production, Symmetry Columns offer confidence in your results, providing the same level of precision day after day.

One of the standout features of Symmetry HPLC Columns is their ability to provide superior peak shape, particularly when working with basic analytes. Symmetry Columns are available in a range of chemistries to suit different analytical needs. The Symmetry C18 and C8 are general-purpose, high-efficiency, reversed-phase columns that deliver maximum reproducibility for a broad spectrum of analytes. The Symmetry Shield RP18 and RP8 offer superior peak shapes, particularly for basic analytes at neutral pH, due to their embedded polar group technology. For those working with peptides and proteins, the Symmetry300 C18 and C4 provide wide-pore, reversed-phase chromatography with high recoveries, ensuring high-quality separation and analysis of complex biological samples.

The Symmetry Shield Columns incorporate Waters' patented embedded polar group technology, which shields the silica's residual silanols, resulting in improved chromatographic performance. By placing the polar group near the silica surface, this technology minimizes silanol activity and enhances peak shape and selectivity, even under challenging conditions. This makes Symmetry Shield Columns an ideal choice for basic analytes at neutral pH, delivering sharp and precise peaks that can enhance method development and performance.

For those working with peptides and proteins, Symmetry300 Columns offer specialized performance, particularly in reversed-phase chromatography. The wide-pore design of these columns (300 Å) allows for the effective separation of both hydrophilic and hydrophobic peptides, neutral compounds, and highly basic peptides. Each Symmetry300 column is quality-control tested with peptide samples to ensure high batch-to-batch reproducibility, making them particularly useful for applications that require the highest peptide recovery. This testing guarantees reliable results, even with high or low mass loads, ensuring you can maintain peak performance in your peptide and protein analyses.

Symmetry HPLC Columns from Waters are an essential tool for anyone seeking reproducibility, peak performance, and reliability in their chromatographic analyses. Whether you are working with small molecules, peptides, or proteins, Symmetry Columns offer the performance and precision necessary to achieve consistent and reproducible results, making them a trusted choice in the pharmaceutical and biopharmaceutical industries.

FAQs About Waters Symmetry HPLC Columns

1. What is the composition of Waters Symmetry HPLC Columns?
Waters Symmetry HPLC Columns are made of silica-based stationary phases with a wide range of chemistries, including C18, C8, Phenyl, and C4.

2. What is the recommended pH range for using Waters Symmetry HPLC Columns?
Waters Symmetry HPLC Columns are recommended for use in the pH range of 2-8.

3. What is the maximum operating temperature for Waters Symmetry HPLC Columns?
The maximum operating temperature for Waters Symmetry HPLC Columns is 60°C.

4. What is the particle size of Waters Symmetry HPLC Columns?
Waters Symmetry HPLC Columns are available in particle sizes of 3.5 µm, 5 µm, and 7 µm.

5. What applications are Waters Symmetry HPLC Columns suitable for?
Waters Symmetry HPLC Columns are suitable for a wide range of applications, including pharmaceuticals, environmental analysis, food and beverage, and clinical research.