XTerra Columns

XTerra Columns

Optimal selectivity at any pH for more robust separations

Optimal selectivity at any pH for more robust separations

Historically, limitations of the stationary phase material have imposed restrictions on speed, resolution, pH, temperature, and loading capacity for scientists doing HPLC separations. XTerra HPLC Columns retain the advantages of silica without restrictions on speed, resolution, pH/temperature instability and loading.

XTerra Columns, which use the first generation of Hybrid Particle Technology (HPT), broke these boundaries and enabled scientists like you to realize the full potential of analytical and preparative separations.

Resolve your toughest separation challenges with XTerra Column chemistries, which include C18, C8, and Phenyl chemistries providing a wide range of selectivity.

XTerra HPLC Column family XTerra Columns
Shop Now

Specifications

Overview

  • Achieve longer column lifetimes at high pH, pH >8.0
  • Get robust separations for basic compounds at high pH
  • Reduce surface silanols for exceptionally sharp, high-efficiency peaks for basic compounds
  • Obtain analytical performance in preparative dimensions with maximum column stability

Recommended Use: For high pH drug discovery, method development, and purification separations.

XTerra Column Chemistries

XTerra Column Chemistries
XTerra MS Columns
  • MS applications
  • High efficiency
  • Wide pH range
  • Chemically stable
  • Long column lifetime

Designed to be compatible with MS applications and provide sharp peaks, good sensitivity, and large peak capacity.

XTerra RP Columns
  • General purpose
  • High efficiency
  • Wide pH range
  • Chemically stable
  • Long column lifetime

Combines hybrid particle technology with shield technology to deliver superior basic analyte peak shape for successful method development.

XTerra Phenyl Columns
  • High efficiency
  • Wide pH range
  • Different selectivity to C18
  • Long column lifetime

Combines hybrid particle technology with phenyl chemistry to give optimal surface coverage, sharp peaks, and reproducibly retention times. Provides complimentary selectivities especially for compounds with aromatic rings.

Ruggedness, efficiency and speed

The Hybrid Particle Technology of XTerra HPLC Columns overcomes the limitations of silica-based and polymer-based stationary-phases by combining the best attributes of both these materials while overcoming each material’s weaknesses. The result is a rugged material that has high mechanical strength, high efficiency, excellent peak shape for bases, and easy scale-up from analytical to preparative chromatography.

Develop robust separations in less time

One of the greatest challenges facing method development scientists is creating a robust separation in the most time-efficient manner possible. XTerra Columns have a usable pH range (pH 1-12) that is twice as wide as silica-based LC columns, making method development twice as easy and twice as fast.

Direct scale-up capability

Combining the patented Optimum Bed Density (OBD) design with the unique chromatographic properties of Hybrid Particle Technology enables XTerra Prep OBD Chromatography Columns to deliver analytical performance in preparative dimensions with maximum column stability. With these capabilities, new drug candidates can go directly from the lead generation to the lead optimization stage of drug discovery. The ability to operate at high pH provides excellent peak shapes for bases and increased mass loading (upto 60x) as compared to conventional silica-based materials.

Stay compliant with smart column options

Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (3 µm particles) used with Alliance iS HPLC System that is optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.

Resources

Documents

Documents

Support

Support

What do you want to do?

What do you want to do?
Need Help?

Related

VanGuard Column Protection
Prolong analytical column lifetime while maintaining chromatographic performance with VanGuard Pre-columns and cartridges.

Sample Vials
Reduce failed assays with Waters glass and plastic autosampler vials and high sample recovery vials - certified/tested by HPLC, GC, and MS.

Quality Control Reference Materials
Ensure peak LC and LC-MS column and system performance by benchmarking with Waters Quality Control Reference Materials, a portfolio of ready-to-use standards and mixes.

Offers and Discounts

Back To Top Back To Top
XTerra Columns

When it comes to high-performance liquid chromatography (HPLC), Waters' XTerra Columns stand out as a paragon of innovation, offering unparalleled robustness and versatility. Designed with the pioneering Hybrid Particle Technology (HPT), these columns address the previous limitations faced by chromatographers, including constraints on speed, resolution, pH, temperature, and loading capacity.

Traditional silica-based columns often falter under extreme conditions of pH and temperature, limiting their application in complex separations. XTerra Columns, however, excel where those others fall short. By retaining the advantageous properties of silica and mitigating its limitations, these columns enable enhanced speed, resolution, and stability across a broad pH range (1-12). This capability is twice the range typical of conventional silica-based LC columns, making XTerra an ideal choice for method development that is both swift and effective.

At the heart of XTerra Columns is the first generation of Hybrid Particle Technology. This innovative approach combines the best features of silica-based and polymer-based stationary phases. The result is a rugged, mechanically strong material that exhibits high efficiency and exceptional peak shapes, particularly for basic compounds. The columns are designed to facilitate easy scale-up from analytical to preparative chromatography, a crucial factor in accelerating the pace of drug discovery and development.

The columns are engineered to withstand high pH conditions, significantly extending their operational lifetime. This durability is paired with the capability to maintain robust separations and reduce surface silanols, which commonly interfere with peak shapes. Furthermore, XTerra's design incorporates Optimum Bed Density (OBD), enhancing the analytical performance in preparative scale applications and ensuring maximum column stability.

XTerra Columns come in various chemistries, including C18, C8, and Phenyl, each with unique selectivity to tackle the most challenging separations. This versatility ensures that chromatographers can achieve sharp, high-efficiency peaks, especially for basic compounds. The columns are especially suited for high pH applications, such as drug discovery, where maintaining column integrity and performance at pH levels above 8.0 is paramount.

Waters also offers smart column options to enhance traceability and compliance in regulated environments. The eCord and eConnect column tags, available for different particle sizes and systems, are designed to record and store detailed column usage data. This information can be accessed through compatible HPLC systems, offering a convenient method for tracking column history and facilitating troubleshooting.

Waters' XTerra Columns redefine the boundaries of chromatographic separation with their superior design and technology. By allowing direct scale-up, operating across a wide pH range, and offering smart technology options, these columns provide a comprehensive solution that meets the rigorous demands of modern chromatography labs. Whether for analytical or preparative applications, XTerra Columns empower scientists to develop more robust, efficient, and time-saving methods, driving forward the fields of pharmaceuticals, biotechnology, and analytical research.