RapiZyme Proteinase K Digestion Module
Achieve faster, more efficient nucleic acid analyses
From R&D to product manufacturing, gene therapies need to be comprehensively analyzed to ensure accurate, efficacious, and safe dosing. The nucleic acid content of a viral vectored gene therapy needs to be tested, and synthetic oligonucleotide therapies must be carefully studied for pharmacokinetic, pharmacodynamic, and metabolism information.
Current assays cannot always effectively support sensitivity and accuracy requirements by regulators or leverage simple to implement sample preparation steps. Accessing the nucleic acid content from relevant sample and minimizing the interference of protein components is challenging, and the Waters RapiZyme Proteinase K Digestion Module is ideal for just that.
The Waters RapiZyme Proteinase K Digestion Module is a ready to use, all-inclusive sample preparation kit containing three easy-to-use solutions for non-specific enzymatic degradation of proteins and improved workflow efficiency. The kit is certified to be free of exonucleases, endonucleases, and ribonucleases for high quality, accurate, and precise nucleic acid measurements. With the RapiZyme Proteinase K Digestion Module, your lab can easily prepare a protein-digested nucleic acid sample in as little as 40 minutes.
RapiZyme Proteinase K Digestion Module
Specifications
Overview
- Perform non-specific enzymatic degradation of proteins for the efficient analysis of gene therapeutics in as little as 40 minutes with an easy-to-use, all-inclusive kit
- Facilitate the extraction of nucleic acid analytes from protein-containing samples when performing DMPK and ADME studies
- Disrupt protein binding that can interfere with the extraction of oligonucleotides from biofluids
- Release viral vectored genomes during the characterization of vectored gene therapies for seamless follow-up analyses
Recommended Use: For performing non-specific enzymatic degradation of proteins when analyzing gene therapeutics.
Improve sample preparation speed using an all-inclusive kit
The RapiZyme Proteinase K Digestion Module comes ready to use and contains three easy-to-use solutions for efficient non-specific enzymatic degradation of proteins:
- RapiZyme Proteinase K has a sequence derived from T. album and is recombinantly expressed and purified from P. pastoris. Certified to be free of exonucleases, endonucleases, and ribonucleases, the solution ensures high quality, accurate, and precise nucleic acid measurements.
- As a subtilisin-type serine protease, RapiZyme Proteinase K exhibits optimal enzyme activity between 50–60 ℃, is active between pH 4 and 12, and is highly compatible with denaturants and surfactants such as 1M guanidine and 0.5% SDS.
- The Guanidine Tris Buffer Solution is a neutral pH solution of concentrated guanidine hydrochloride.
- The TCEP HCl concentrate is a stable solution format of a phosphine-based reducing agent solution.
Quick and Efficient Protein Digestion
The RapiZyme Proteinase K Digestion Module has been performance-tested for its ability to degrade plasma proteins in under 40 minutes. The accompanying chart shows an incubation time course for the digestion of plasma proteins at a 55 ℃ temperature in the presence of guanidine hydrochloride and TCEP reducing agent. SEC profiles are provided which demonstrate a shift of sample composition from large proteins to small peptides.
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