• Application Note

Theophylline and Related Compounds

Theophylline and Related Compounds
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of theophylline and related compounds using Symmetry Shield RP18 Columns. 

Introduction

The compounds analyzed in this study are:

  1. Theobromine 
  2. Theophylline 
  3. 7-(2-hydroxyethyl) Theophylline 

Experimental

HPLC Method

Column:

Symmetry Shield RP18, 3.0 x 150 mm, 5 μm

Part number:

186000692

Mobile phase:

5 mM potassium phosphate, pH 2.0/acetonitrile 95:5

Flow rate:

0.6 mL/min

Injection volume:

5 μL injection of solution containing theobromine (0.22 mg/mL), theophylline (0.26 mg/mL), and 7-(2-hydroxyethyl) theophylline (0.13 mg/mL)

Detection:

UV @ 270 nm

Results and Discussion

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WA31763.169, June 2003

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