What is non-specific adsorption (NSA)? NSA refers to adsorption that was not intended between your sample and a surface. It is an undesired chemical interaction based predominantly on ionic interactions. Electron-rich compounds, such as those containing phosphate or carboxylate groups, organic acids, metal chelating compounds, and more can be lost to non-specific adsorption on metal surfaces.
Metal-sensitive compounds span a variety of compounds in biopharmaceutical, small molecule pharmaceutical, food/environmental, materials, and biomedical research applications including, organic acids, organophosphates, oligonucleotides, phosphopeptides, acidic peptides, acidic phospholipids, acidic glycans, nucleic acids, and metal chelating compounds.
These metal-sensitive analytes stick to the metal surface and cannot be removed. To prevent this problem, you either need to maintain the environment so that the interactions are not strong (through the use of complex mobile phases, conditioning, passivation, or other time-consuming and non-robust methods), or avoid the interaction altogether. MaxPeak High Performance Surfaces (HPS) Technology was specifically designed to prevent these undesired metal interactions within the flow path of the LC system.
The inert hardware is comprised of an inorganic-organic hybrid metal treatment that is non-reactive with compounds that interact with metal surfaces. The idea is to stop acidic groups from undergoing Lewis acid/base interactions with electron-deficient metal ions coming from the separation path in the workflow. MaxPeak Premier Columns feature this inert hardware, resulting in improved peak shape, improved recovery and sensitivity, superior reproducibility, and stable performance.
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