Waters workflow solutions deliver quick and accurate intact protein measurement for confirmation of identity, modifications, and glycoform heterogeneity, and include:
Webinar: SEC Column Selection and Care for Biotherapeutic Proteins and Peptides
Achieve rapid and confident decisions driven by accessible, high-quality data with the BioAccord LC-MS System with ACQUITY Premier that is specifically designed for routine monitoring in biopharmaceutical process and product development.
Maximize sample information for your analytes from detailed characterization to accurate quantitation with the Xevo G3 QTof, which enables complete confidence in your results – from challenging small molecules to complex biotherapeutics.
Unlike competitor systems with restricted inlet options, scan function limitations, or requiring multiple platforms, only Waters offers an all-encompassing high-performing LC-MS solution, the SYNAPT XS mass spectrometer, for ultimate flexibility and greater freedom of analytical choice – supporting scientific creativity and technical success for any application.
Developed through focused collaboration, the SELECT SERIES Cyclic IMS combines novel cyclic ion mobility separation with state-of-the-art, high performance, time-of-flight mass spectrometry, enabling leading researchers to unlock the potential in scientific discovery.
Eliminate the unpredictability of analyte losses due to metal interactions with MaxPeak Premier Solutions that combine state-of-the-art column and system technology for maximum sensitivity and reproducibility.
Place downstream of any LC and perform advanced analysis of protein-complexes, post-translational modifications, and aggregation with the DAWN MALS Detector that assists at every development stage. Couple with Eclipe to measure binding affinity during discovery.
Combine DLS, SLS, and ELS all in one platform with the DynaPro ZetaStar instrument, specifically designed for biopharma to measure zeta-potential in formulation buffer without dilution.
Measure size, polydispersity, and Tagg data for hundreds of samples with as little as 4 µL per well with the DynaPro DLS Plate Reader that uses standard-well plates and can integrate with any liquid handler to boost productivity further.
Directly monitor titer, size, and impurities in real-time with ultraDAWN Multi-angle Light Scattering Detector that seamlessly connects downstream purification and enrichment methods, such as IEX, FPLC, or TFF.
Analysis of NISTmAb reference material 8671 on the XBridge Premier Protein SEC 250 Å and the competitive silica dSEC-2 column Isocratic separation performed using a mobile phase of 2x PBS in water, flow rate of 0.57 mL/min, and UV detection at 280 nm.
Using the UPLC Intact Mass Analysis Kit, rapid gradient cycles were applied to regenerate the column to pre-injection conditions as part of the analysis of an intact antibody. The total ion chromatogram (TIC) shows no detectable carryover following a 0.5 µg injection. Thus, the need to use inter-run blank injections with difficult samples is eliminated.
Peptide mapping analysis of trastuzumab fractions. (A) Trastuzumab fractions collected from a BioResolve SCX mAb, 4.6 × 100 mm Column. (B) Mirror image of peptide maps for Fractions 5 and 7. A peak at a retention time of 30.24 min increased significantly in Fraction 5, compared to that in Fraction 7 (indicated by the blue arrow). (C) Extracted ion chromatograms (XIC) for identified peptides. The peak at 30.26 min has been identified as deamidation of the peptide ASQDVNTAVAWYQQKPGK. The un-modified peak has a retention time of 29.63 min. (D) MS-MS fragment comparison of the deamidated and un-modified peptide ASQDVNTAVAWYQQKPGK. The fragments that do not contain asparagine have the same mass value, while the fragments that do contain asparagine differ in 1 Da in mass.
Combined MS spectra, zoom, and corresponding MaxEnt1 deconvoluted masses of trastuzumab using different IDC counts.
Separation of mAb Subunit Standard using 1-minute and 4-minute gradient on a 20 mm column, as well as 20-min gradient on a 100 mm column. Comparable combined spectra of the three main peaks and the deconvoluted mass (insert) were obtained.
Comparison of LC-MS separation of the mAb Subunit Standard on a 2.1 x 20 mm BioResolve Premier RP mAb Polyphenyl 450 Å 2.7 µm Column (4-minute gradient) and a 2.1 x 100 mm BioResolve RP mAb Polyphenyl 450 Å 2.7 µm Column (20-minute gradient).
