Purification Sample Management

Purification Sample Management

Comprehensive and flexible solutions for HPLC sample management

Comprehensive and flexible solutions for HPLC sample management

From high performance liquid chromatography (HPLC) sample aspiration and injection to fraction collection, Waters provides easy-to-use sample management options.

Waters offers an array of easy-to-use advanced sample managers to accommodate automated purification systems, including the 2707 Autosampler, 3767 Sample Manager (Injector/Collector), Manual FlexInject Module, Manual Prep Injector Kit, and Preparative Chromatography Rack, for flexible, easy-to-use purification sample management.

2707 Sample Manager Purification Sample Management

Overview

  • 2707 Autosampler – A flexible autosampler that manages sample injection from both microtiter plates and/or vials. It can also be configured for biocompatible or preparative scale injections.
  • 3767 Sample Manager (Injector/Collector) – A high-capacity sample processing system that easily and automatically manages concurrent sample injection, collection of peak fractions, and fraction analysis on a single HPLC or LC-MS platform.
  •  Manual FlexInject Module – The FlexInject includes analytical and preparative valves with a manual selector valve. It features the reliable 7725 analytical injector with a 100 µL sample loop and the 3725i preparative injector configured with a 5 mL sample loop. Additional loop sizes can be ordered separately.
  • Manual Prep Injector Kit – The Prep Injector was built on the Flex Inject platform. It features the reliable 3725i preparative injector comes standard configured with a 5 mL sample loop and a 10 mL sample loop in the start-up kit. Additional loop sizes can be ordered.
  • Preparative Chromatography Rack – The Waters Preparative Chromatography Rack (PCR) incorporates manual small-scale and large-scale injectors with manual fraction collection. You can use the scale select valve to alternate between the small-scale and large-scale flow paths.
  • Waters Fraction Manager – Efficiently isolate and recover small samples for additional analyses and increase recovery with the Waters Fraction Manager, a fraction collector designed for use with UPLC systems.
  • Waters Fraction Collector III – Precisely perform fraction collection in chromatography with the easy-to-use Waters Fraction Collector III, which can be used alone or as part of a Waters sample purification system for even greater sample collection capacity.

Recommended Use: For flexible, easy-to-use purification sample management.


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2707 Autosampler

The 2707 Autosampler is a versatile, compact sample management system that makes highly precise and reproducible injections of samples into your LC or LC-MS system. It is ideally suited for laboratories where reduced carryover, maximized repeatability, high accuracy, and application flexibility are the primary focus.

Features:

  • Compact and easy to service design
  • High-resolution syringe control for high-precision injections
  • Interchangeable fixed-volume sample loops
  • Variable-volume partial-loop injection capability
  • Optional sample cooling for consistent results
  • Pressure-assisted sample aspiration injection capability
  • Use of plates or vials, alone or in combination, for varied sampling formats
  • Empower and ChromScope v2.1 Software control

3767 Sample Manager (Injector/Collector)

The Waters 3767 Sample Manager is a high-capacity sample processing system that effortlessly and automatically manages concurrent sample injection, collection of peak fractions, and fraction analysis on a single HPLC or LC-MS platform. Intended for applications involving analytical and preparative scale purifications, the 3767 is controlled by Waters MassLynx Software and compatible with the LC Prep AutoPurification System and the SFC Prep 150 AP System.

Features:

  • With its diverse compatibility, a large variety of sample formats (test tubes, microtiter plates, and vials) can be used for injection and collection
  • Separate analytical and preparative injection valves, allowing you to alternate between analytical, and preparative scale isolation and purification
  • Optional fume hood accessory provides an outlet for localized ventilation of hazardous vapors
  • High-capacity single platform (up to 15 96-well microtiter plates; 544 13 x 100 mm test tubes; 720 2 mL vials) for injection and collection
  • Collect sample fractions sequentially or with one-for-one mapping
  • High velocity wash pumps that quickly wash your probes

Manual Prep Injector Kit

The Manual Prep Injector Kit features the same reliable 3725i preparative injector that comes standard configured with a 5 mL sample loop and a 10 mL sample loop in the start-up kit. Additional loop sizes can be ordered separately. Use either the partial-filling or the complete-filling method options for sample loop loading.

Features:

  • Continuous flow during fluid path connection for error-free changeover
  • Convenient internal position switch provides a start signal for data stations or auto-zero for detectors
  • Easy access to fittings with wide port angles

FlexInject Manual Dual Injector Module

The FlexInject Manual Dual Injector Module operates with any fluid mixing system, including Waters high-pressure mixing solvent delivery modules (2545 Binary Gradient Module or 1525EF Binary HPLC Pump).

Features:

  • Inject small or large sample volumes by simply selecting a valve
  • Select an injector and column appropriate for your sample size
  • Runs at flow rates of up to 150 mL/min
  • Easy operation and maintenance
  • Mount the module on either side of the delivery systems for easy access

Preparative Chromatography Rack

The Waters Preparative Chromatography Rack (PCR) incorporates manual small-scale and large-scale injectors with manual fraction collection, enabling you to alternate between the small-scale and large-scale flow paths using the scale select valve.

Features:

  • Inject small or large sample volumes by simply selecting a valve
  • Select an injector and column appropriate for your sample size, from 3.9 mm to 50 mm
  • Runs at flow rates up to 150 mL/min
  • Manually collect up to 8 fractions

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HPLC Sample Management constitutes a comprehensive array of procedures and methodologies designed to ensure the effective and precise handling of samples throughout the process of high-performance liquid chromatography (HPLC) purification. This fundamental facet of chromatography involves a sequence of crucial steps:

Sample Preparation: Prior to commencing the purification procedure, it is essential to adequately prepare samples. This involves guaranteeing proper dissolution of samples in the suitable solvent and filtration to eliminate any particulate matter that might disrupt the HPLC analysis.

Column Selection: The selection of the appropriate HPLC column is pivotal for successful purification. The column's stationary phase, dimensions, and particle size must be chosen based on the specific characteristics of the sample undergoing purification.

Method Development: The formulation of an effective purification method requires the optimization of parameters such as mobile phase composition, gradient conditions, and flow rates. This step ensures the separation of the target compound from impurities with high resolution.

Fraction Collection: Throughout the purification process, the HPLC system segregates sample components based on their affinity for the stationary phase. Fraction collection involves the gathering and storage of eluted fractions containing the purified compound.

Aspiration and Transfer: Following the collection of fractions, it is imperative to aspirate them from the collection vessels. Meticulous handling and transfer of purified samples are necessary to prevent contamination and ensure the integrity of collected fractions.

Sample Injection: Upon completion of the purification process, the purified sample is introduced into the HPLC system for further analysis or downstream applications. Employing proper injection techniques is crucial for maintaining the accuracy and reproducibility of results.

Data Management: The recording and management of data generated during the purification process are crucial for traceability and quality control. This encompasses documenting key parameters such as injection volume, flow rates, and fraction collection times.

Instrument Calibration and Maintenance: Routine calibration and maintenance of the HPLC system are essential to ensure its proper functioning. This involves verifying detector sensitivity, checking flow rates, and replacing consumables to prevent issues that could compromise sample purity.

Quality Control: The implementation of quality control measures, such as running standard reference samples, aids in validating the efficiency and reproducibility of the purification process. This step ensures that purified samples meet the required quality standards.

User Training and Documentation: Providing adequate training to personnel involved in the purification process is crucial to minimize errors and optimize efficiency. Additionally, maintaining comprehensive documentation of procedures and results is essential for compliance with regulatory requirements and for troubleshooting purposes.

In summary, "Purification HPLC Sample Management" entails a meticulously orchestrated series of steps, spanning from sample preparation to data management, with the goal of achieving precise and reliable purification of target compounds through high-performance liquid chromatography.