ACQUITY UPLC H-Class PLUS System

ACQUITY UPLC H-Class PLUS System

Performance that advances your lab

Performance that advances your lab

Whether your goal is to rapidly develop new methods or to routinely analyze large numbers of incoming samples, the ACQUITY UPLC H-Class PLUS System can help you decrease the time to result, while increasing the depth and quality of information received from your sample analysis.

With the ACQUITY UPLC H-Class PLUS System, you can improve laboratory productivity by transitioning methods to UPLC/UHPLC technology, improve the characterization of your most complex samples through enhanced resolution and leverage advanced tools to simplify and streamline your method development workflow.

Simplify and streamline your method development workflow. The ACQUITY H-Class PLUS Quaternary System automatically screens multiple column and eluent combinations to identify the separation conditions that provided the best chromatographic resolution. The system provides on-demand solvent blending and support for any analytical LC column, making it an ideal choice for modernizing existing or developing new methods. ACQUITY UPLC H-Class PLUS System

Specifications

Maximum operating pressure

15,000 psi

pH range*

1–12.5

Operating flow rate range

Up to 2.2 mL/min

Sample flow path

Stainless-steel

Sample carryover

≤0.002% (Caffeine) under UV conditions

Sample compartment

4–40 ˚C

Pump options

Binary, Quaternary

Solvent conditioning

Active Pre-heating

Column tracking

eCord Technology tracks column information and archives column usage

Column capacity

Up to 6 columns of 150 mm length and up to 4.6 mm I.D.

Maximum sample capacity

Up to 20 plates (with Sample Organizer)

Detector options

PDA Detector

TUV Detector

FLR Detector

RI Detector

ELS Detector

Mass Spectrometers

External control

Empower Software

MassLynx Software

waters_connect

UNIFI Scientific Information System

Physical specifications

(QSM or BSM, SM-FTN, CHA)

Width: 34.3 cm (13.5 in.)

Height: 71.1 cm (28.0 in.)

Depth: 71.2 cm (28.0 in.)

* For specific test conditions, contact your Waters sales representative

Overview

  • Get the flexibility of multi-solvent blending - Blend up to nine solvents with integrated solvent-select valve with the Quaternary Solvent Manager 

  • Deliver precise and accurate binary solvent blending - Blend up to four solvents with integrated solvent-select valve with the Binary Solvent Manager

  • Get high-precision injections with direct inject sampling - The needle-in-flow path design ensures an accurate needle seal at high pressure

  • Get accurate temperature control across the entire column - Configurable for 1 to 6 columns with active preheating

  • Automate the formulation of mobile phases - Significantly reduce the time you spend in the prep lab with  Auto•Blend Plus Technology

  • Transfer methods more easily - Easily adjust for system volume differences when transferring methods from an alternative LC system without alteration to the method table using Gradient SmartStart 

  • Wide software support – ACQUITY UPLC H-Class PLUS System is supported by  Empower, MassLynx, UNIFI, and several 3rd party software packages

Recommended Use: Improving laboratory productivity by transitioning methods to UPLC technology.

Discover the UPLC difference

The ACQUITY UPLC H-Class PLUS System is the next evolution of ultra-performance instrumentation, providing the high resolution of a quaternary or binary liquid chromatography (LC) system.

Achieving true UPLC performance requires that system dispersion (or extra-column band spread) to be matched to the peak widths associated with narrow bore columns packed with sub-2-μm particles. While other systems might be designed for high pressure, it doesn’t mean they're designed to deliver the sharp peaks of UPLC performance and the ACQUITY UPLC H-Class PLUS System.

Take your methods to the next level

Leverage the benefits of UPLC technology to improve your existing HPLC methods. By scaling your methods to UPLC, your laboratory will benefit from the combination of increased resolution, sensitivity, and speed that comes with modern small particle chemistries.

Designed to accommodate all of your LC method needs, the ACQUITY UPLC H-Class PLUS System is ideally suited to future-proof your laboratory, enabling the support of existing HPLC methods now, and provide significant productivity and cost benefit when utilized for UPLC methods. 

Discover the best method for the best results

Get the versatility to discover the best set of chromatographic conditions for your sample, providing a more robust method. The flexibility of the ACQUITY UPLC H-Class PLUS Quaternary System allows you to automatically screen important method parameters, including:

  • Up to six columns in independently temperature-controlled compartments

  • pH and ionic strength with Auto•Blend Plus Technology

  • Optional and integrated solvent selection valve expands access to six additional mobile phase lines 

  • 11 unique gradient profiles

Deliver greater speed and precision for routine analyses

The ACQUITY UPLC H-Class PLUS Binary System combines the proven reliability and performance of the ACQUITY UPLC H-Class PLUS System with the precision and lowest system volume offered by our high-pressure mixing binary solvent manager, allowing you to:

  • Accommodate the most challenging sample matrices

  • Tackle applications with long shallow gradients

  • Improve the throughput of your methods 

Combine the capabilities of the ACQUITY UPLC H-Class PLUS Binary System with Waters Tandem quadruple technology  for routine quantitative UPLC-MS/MS applications, so you can confidently quantify and trace components in the most complex of samples.

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The ACQUITY UPLC H-Class PLUS System, developed by Waters Corporation, establishes a leading standard in liquid chromatography equipment, seamlessly integrating advanced technology and innovative design to meet modern laboratory needs.

Fundamentally, this system is engineered to provide exceptional performance, reliability, and flexibility in chromatographic analyses. It combines high-pressure solvent delivery, precise sample injection, and sensitive detection capabilities to achieve outstanding resolution, sensitivity, and throughput.

Key features of the ACQUITY UPLC H-Class PLUS System include its capacity to operate at ultra-high pressures, facilitating swift separations and increased sample throughput without compromising resolution or peak capacity. These attributes result from innovative design enhancements such as optimized flow paths and advanced solvent delivery mechanisms.

Moreover, the system offers remarkable adaptability, catering to a broad range of applications in industries like pharmaceuticals, biotechnology, and environmental analysis. Its modular design enables seamless integration with other analytical instruments and automation platforms, boosting workflow efficiency and productivity.

Supported by robust data acquisition and processing software, users benefit from intuitive tools for method development, optimization, and data analysis. Whether conducting routine analyses or complex research projects, the system provides a user-friendly interface and comprehensive functionalities to streamline chromatographic workflows.

In conclusion, the ACQUITY UPLC H-Class PLUS System represents the epitome of liquid chromatography technology, empowering laboratories to achieve superior results and drive scientific innovation across diverse applications and industries with its exceptional performance, reliability, and adaptability.

FAQs About ACQUITY UPLC H-Class PLUS System

1.How does the quaternary solvent manager enhance performance?
The quaternary solvent manager in the ACQUITY UPLC H-Class PLUS System allows precise blending of up to four solvents, enabling complex gradient elutions and improved separation efficiency. This flexibility facilitates the optimization of methods and enhances the reproducibility of results, making it easier to handle a wide range of sample types and analytical requirements.

2.What maintenance is required for the ACQUITY UPLC H-Class PLUS System?
Routine maintenance for the ACQUITY UPLC H-Class PLUS System includes regular cleaning of the solvent lines, degasser, and sample manager. It is also important to periodically check and replace pump seals, pistons, and filters to ensure consistent performance. Following the recommended maintenance schedule helps to prolong the system"s lifespan and maintain optimal performance.

3.Can the ACQUITY UPLC H-Class PLUS System be integrated with other laboratory instruments?
Yes, the ACQUITY UPLC H-Class PLUS System can be integrated with a variety of laboratory instruments from Waters, including sample preparation units, autosamplers, and detection systems. This integration capability enhances workflow efficiency and enables comprehensive analytical solutions for complex sample analyses.

4.What software does the ACQUITY UPLC H-Class PLUS System use?
The ACQUITY UPLC H-Class PLUS System uses Empower Chromatography Data System (CDS) software. Empower software is known for its comprehensive data management, robust instrument control, and powerful data analysis capabilities. It offers an intuitive user interface that simplifies method development, data acquisition, and reporting.