ACQUITY UPLC M-Class System with HDX Technology

ACQUITY UPLC M-Class System with HDX Technology

Quantify changes in protein conformation with confidence

Quantify changes in protein conformation with confidence

Monitoring protein conformation can be challenging. With the ACQUITY UPLC M-Class System with HDX Technology, your lab can leverage UPLC separations and high-resolution MS (HRMS) to answer important questions about protein conformation through the power of hydrogen deuterium exchange (HDX), like drug candidate binding to a protein, biopharmaceutical product development, protein-protein interactions, and intellectual property.

The system provides HRMS and intuitive informatics for confident measurement of changes in protein conformation by controlling important experimental parameters. With the ACQUITY UPLC M-Class System with HDX Technology, your lab can confidently identify changes in protein conformation by stringently controlling the labeling reaction, and efficiently processing and separating the sample before mass analysis.

ACQUITY UPLC M-Class System with HDX Technology. ACQUITY UPLC M-Class System with HDX Technology

Specifications

Maximum operating pressure  

15,000 psi

pH range

2 to 10

Operating flow rate range  

200 nL/min to 100 μL/min without flow splitting

Sample flow path   

Non-ferrous

Injection volume

50 µL loop standard; 1, 2, 5, 10, 20, 100, 250 µL loops available

Sample compartment  

0–25 °C

Pump options  

Binary

Detector options

PDA Detector

Mass Spectrometers

External control   

MassLynx Software

Physical specifications

Width: 34 cm (14 in.)  

Height: 76 cm (30 in.) 

Depth: 71 cm (28.0 in.)  

Overview

  • Rapid chromatographic resolution at 0 °C with UPLC technology
  • Temperature-controlled environment for trapping and analytical columns
  • Repeatable peptic digestion with Waters Enzymate BEH Pepsin Column, capable of operation up to 15,000 psi
  • Easy access injection port for flexibility with manual or robotics injection
  • Integrated with Waters high resolution MS for precise determination of Deuterium label
  • Intuitive, interactive DynamX HDX Data Analysis Software to shorten the time between experiment and answers

Recommended Use: Answering important questions about changes in protein conformation.

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Features Header

Accurately, reproducibly, and easily perform protein conformation studies

The unprecedented system-level design integrates all the steps necessary to accurately, reproducibly, and most of all, easily, perform protein conformation studies. The system and its DynamX HDX Analysis Software remove the barriers to adopting high resolution LC and MS for routine studies of protein conformational changes, making hydrogen deuterium exchange with MS (HDX, HDX MS, HXMS, or H/D exchange) a core competency for your laboratory.

Higher efficiency separations

Carefully controlling temperature-sensitive parts of the HDX workflow ensures that the conformational changes are captured and not missed. UPLC separations of up to 15,000 psi take place within the cooled environment with the added advantage of sub-2-µm particles for enhanced chromatographic resolution and speed. Maintaining temperatures at 0±1 °C means that back-exchange is kept to an absolute minimum with the ACQUITY UPLC M-Class System with HDX Technology.

Once the HDX reaction has been chemically quenched and manually introduced, the HDX Manager directs the sample to the pepsin column for peptic digestion, or directly into the mass spectrometer (MS) for global analysis. The sample is then trapped for a rapid UPLC analytical separation.

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The ACQUITY UPLC M-Class System, equipped with HDX Technology, is at the forefront of liquid chromatography for precise scientific analysis.

This system excels in numerous aspects. UPLC, or Ultra-Performance Liquid Chromatography, showcases its ability to swiftly achieve high-resolution separations with heightened sensitivity compared to traditional methods. The "M-Class" designation underscores its compact design, making it ideal for space-constrained labs while maintaining outstanding performance.

HDX Technology marks a significant leap in chromatography, enhancing resolution, sensitivity, and peak capacity for intricate separations of complex mixtures. It utilizes advancements in column chemistry, detector sensitivity, and data processing algorithms to deliver superior analytical performance.

ACQUITY UPLC M-Class System with HDX Technology FAQs

1. What is HDX technology?
Hydrogen-deuterium exchange (HDX) technology is a powerful technique used to study protein structure, dynamics, and interactions. In HDX, proteins are exposed to deuterium-containing solutions, allowing hydrogen atoms in the protein to exchange with deuterium. This exchange provides insights into protein conformations and binding sites by revealing regions of structural flexibility and stability.

The ACQUITY UPLC M-Class System is optimized for HDX workflows, offering precise temperature control, minimized back-exchange, and ultra-low dispersion to preserve sample integrity. When paired with mass spectrometry, the system enables high-resolution analysis of labeled peptides, making it ideal for structural biology studies.

2. What flow rate ranges does the system support?
The ACQUITY UPLC M-Class System is specifically designed for microflow separations, supporting flow rates typically in the range of 1–100 µL/min. These low flow rates enhance sensitivity by improving ionization efficiency and reducing solvent consumption, making the system well-suited for HDX experiments and other applications requiring precise control.

The system"s advanced fluidics ensure consistent performance at these low flow rates, delivering high resolution and reproducibility in chromatographic separations.

3. How does the system ensure reproducibility in HDX experiments?
The ACQUITY UPLC M-Class System ensures reproducibility in HDX experiments through its advanced design and automation features. It provides precise temperature control during the entire workflow, preventing unwanted back-exchange and maintaining consistent labeling conditions.

Ultra-low dispersion fluidics preserve the integrity of deuterium-labeled peptides during analysis, while automated sample handling minimizes variability. The system"s compatibility with software like MassLynx and specialized HDX tools further supports consistent data acquisition and processing, ensuring reproducible and reliable results across experiments.

4. Which mass spectrometers are compatible with the ACQUITY UPLC M-Class System with HDX Technology?
The ACQUITY UPLC M-Class System with HDX Technology is compatible with a wide range of high-performance mass spectrometers, including Waters' Synapt series, Xevo TQ and QTof systems, and select third-party instruments.

When integrated with these mass spectrometers, the system enhances sensitivity and resolution by delivering narrow, well-defined peaks and maintaining the integrity of deuterium-labeled peptides.

With applications spanning across various industries, including pharmaceuticals and biotechnology, this system caters to a wide range of tasks, from analyzing small molecules to characterizing large biomolecules like proteins.

Reliability and user-friendliness are paramount, ensuring consistent and reproducible results with minimal user involvement. Its modular design allows seamless integration with other instruments and automation, thereby streamlining workflows and enhancing lab efficiency.

In conclusion, the ACQUITY UPLC M-Class System with HDX Technology sets a new benchmark in chromatography, offering exceptional performance, versatility, and user-friendliness to meet diverse analytical requirements.