ACQUITY UPLC M-Class Chromatography System

ACQUITY UPLC M-Class System

Let your LC inlet do the work for your nano- to micro-scale separations

Let your LC inlet do the work for your nano- to micro-scale separations

Many analytical scientists would like to increase the sensitivity of their LC-MS analyses to meet the demanding goals of their labs, however, adapting conventional separations instruments to improve sensitivity can cause problems with reproducibility and reliability.

The ACQUITY UPLC M-Class System delivers the performance you expect from nano- to microscale separations with the usability of an analytical-scale UPLC system. Each part of the system was designed and tested to deliver the right flow for the right column. The ACQUITY UPLC M-Class System provides excellent performance with overflow rates from 200 nL/min to 100 µL/min and a range of column inner diameter (I.D.) from 75 µm to 1.0 mm.

ACQUITY UPLC M-Class System provides the most reliable nano- to micro-scale separations. ACQUITY UPLC M-Class System

Specifications

Maximum operating pressure  

15,000 psi

pH range

2 to 10

Operating flow rate range  

200 nL/min to 100 μL/min without flow splitting

Sample flow path   

Non-ferrous

Injection volume

0.1 µL to 100.0 µL

Sample compartment  

4–40 ˚C

Pump options

Binary

Column tracking   

None

Column capacity  

Single column, 75 µm to 4.6 mm internal diameter (ID); up to 250 mm in length

Maximum sample capacity

Up to 21 plates (with Sample Organizer)

Detector options

PDA Detector

TUV Detector

Mass Spectrometers

External control   

MassLynx Software

Physical specifications

(µBSM, µSM-FL, TVM)

Width: 34.3 cm (13.5 in.)

Height: 69.7 cm(27.4 in.)

Depth: 71.2 cm (28.0 in.)

Overview

  • System of choice for sample-limited applications in proteomics and biomarker discovery
  • Higher sensitivity for any mass spectrometer (MS)
  • Maximum amount of data from any sample
  • Less sample preparation volume and cost
  • Reduced solvent consumption
  • Increased dynamic range
  • Flexible configurations for HDX technology, 2D, and UV detection

Recommended Use: Meeting usability, sensitivity, robustness, and reproducibility requirements for nano- to micro-scale UPLC separations.

Features Header

Features Header

The analysis you’ve always desired for nano- to microscale separations

The ACQUITY UPLC M-Class System delivers the most robust, reliable, and highest quality nano- to microscale UPLC separations to every laboratory performing LC-MS analyses. With unprecedented usability, the ACQUITY UPLC M-Class System enables laboratories to succeed in analyzing compounds that are severely limited in amount or availability, even amid a complex matrix over several orders of magnitude. 

Confidently achieve your laboratory goals with high-efficiency ACQUITY UPLC M-Class Columns ranging from 75 µm to 1 mm I.D. by using complementary mass spectrometry to support our full range of column inner diameters or run 300 µm to 1 mm I.D. columns with optical detection. The system also offers a range of flexible configurations for HDX technology, UV detection, and 2D analysis.

Your inlet to MS

The ACQUITY UPLC M-Class System expands the performance of any nano- to micro-scale MS analysis. It increases the linear dynamic range of your analysis, resulting in better MS analyses by data-dependent acquisition (DDA) or MSE. The direct (non split) automatic solvent flow control algorithms of the ACQUITY UPLC M-Class System provide pulse-less flow for increased retention time reproducibility. Sample integrity is maintained due the ACQUITY UPLC M-Class System’s non-reactive surfaces. New drivers for MassLynx Software integrate the system seamlessly into your existing workflows.

Monitor protein conformation with HDX technology

The ACQUITY UPLC M-Class System with HDX Technology can quantify small changes in protein conformation by extending its pressure range to enable a higher efficiency separation. The system allows for manual sample injection and integrates the steps of hydrogen-deuterium exchange (HDX) from sample prep to actual sequence determination, D2 uptake curves, and comparison plots with the interactive, highly innovative DynamX HDX Data Analysis Software. HDX data can be used to make business decisions about drug candidate binding to a protein target, biopharmaceutical product development, protein-protein interactions, and intellectual property.

Expand the dynamic range of your analysis with 2D separations

The ACQUITY UPLC M-Class System with 2D Technology has streamlined 2D-LC separations with a highly intuitive menu-driven method setup, standardized separation chemistries, and intelligent valve operation. Expanding on the use of a novel 2D reversed-phase/reversed-phase (RP/RP) approach, where the first dimension is run at pH 10 and the second at pH 2, grants the ability to robustly increase sample throughput with MS-compatible eluents.

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The ionKey/MS System integrates the microflow UPLC separation directly into the source of the mass spectrometer with the iKey. This delivers LC-MS system performance and sensitivity that cannot be achieved any other way.

ACQUITY UPLC M-Class System with HDX Technology
Monitor protein conformation with HDX technology. The ACQUITY UPLC M-Class System with HDX Technology leverages UPLC separations and high-resolution MS to answer important questions about changes in protein conformation.

ACQUITY UPLC M-Class System with 2D Technology
For protein identifications, quantification, and sequence coverage, 2D technology employs reversed-phase chromatography at pH 10 in the first dimension, followed by reversed-phase chromatography at pH 2 in the second dimension.

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The ACQUITY UPLC M-Class System is a high-performance liquid chromatography (HPLC) system designed for microflow LC applications. It offers exceptional sensitivity, speed, and resolution, making it ideal for demanding analytical challenges in various fields such as pharmaceuticals, biotechnology, environmental science, and more.

Key features of the ACQUITY UPLC M-Class chromatography system include:

Microflow LC Capability: The System excels in microflow LC, enabling high-sensitivity analysis with minimal sample usage, ideal for applications requiring precise measurements from small sample volumes.

High Resolution: With its advanced chromatographic capabilities, the ACQUITY UPLC M-Class System delivers high-resolution separations, enabling the detection and quantification of complex sample components with unparalleled precision.

Speed and Efficiency: The system's innovative design and technology enable rapid separations, reducing analysis time and increasing laboratory throughput without compromising data quality.

Flexibility and Versatility: It offers a wide range of chromatographic options, including reverse-phase, ion-exchange, size exclusion, and hydrophilic interaction chromatography, allowing users to adapt methods to suit specific application requirements.

Compatibility and Integration: The ACQUITY UPLC M-Class System is compatible with a variety of detectors, including UV, fluorescence, mass spectrometry (MS), and evaporative light scattering detectors (ELSD), offering versatility in detection options. Moreover, it seamlessly integrates with our Empower Chromatography Data Software, providing comprehensive data management and analysis capabilities.

Overall, the ACQUITY UPLC M-Class Chromatography System is a versatile and reliable chromatography platform that empowers researchers and analysts to achieve superior results in their analytical workflows, whether in academic research, pharmaceutical development, or quality control applications.

ACQUITY UPLC M-Class Chromatography System FAQs

1. What applications are best suited for the ACQUITY UPLC M-Class System?
The ACQUITY UPLC M-Class System is ideal for applications requiring high sensitivity and resolution in nano- to microscale liquid chromatography. These include proteomics, metabolomics, and biomarker discovery, where detecting low-abundance analytes is critical. It is particularly suited for research in pharmaceuticals, biotechnology, and environmental analysis, as well as clinical studies. The system"s precise flow control and advanced separations make it a preferred choice for analyzing complex biological samples and performing targeted or discovery-based workflows in omics research.

2. How does the M-Class System enable nano- to microscale separations?
The ACQUITY UPLC M-Class System is designed with ultra-precise flow control and optimized system components to facilitate nano- to microscale separations. It features low-dispersion tubing, specialized injectors, and nano-scale columns that support ultra-low flow rates, typically in the range of nanoliters to microliters per minute.
This configuration minimizes sample and solvent use while maximizing sensitivity and resolution. By reducing dispersion and improving chromatographic performance, the system ensures high-efficiency separations for small sample volumes and low-concentration analytes.

3. Can the M-Class System integrate with mass spectrometry platforms?
Yes, the ACQUITY UPLC M-Class System is fully compatible with Waters mass spectrometry platforms and other MS technologies. This integration enables seamless coupling of high-resolution nano- or microscale separations with advanced MS detection for comprehensive analyte profiling. The system"s precise flow control and low-dispersion design enhance the sensitivity and accuracy of MS analyses, making it particularly effective for proteomics, metabolomics, and complex sample studies. This combination provides a powerful tool for identifying and quantifying low-abundance analytes.

4. Does the system support automated workflows?
The ACQUITY UPLC M-Class System supports automated workflows through compatibility with advanced software platforms like MassLynx and UNIFI. These platforms enable automated method setup, data acquisition, and analysis, reducing manual intervention and increasing throughput. Features such as autosamplers and scheduled maintenance alerts streamline operations further.