ACQUITY UPLC Systems with 2D LC Technology

ACQUITY UPLC Systems with 2D LC Technology

The lowest dispersion system for maximum peak capacity

The lowest dispersion system for maximum peak capacity

Solve complex UPLC/UHPLC separation challenges with the ACQUITY UPLC Systems with 2D LC Technology, a family of systems and proven columns that offer the lowest dispersion for maximum peak capacity.

Waters 2D technology solutions for the ACQUITY UPLC M-Class System, ACQUITY UPLC I-Class PLUS, and ACQUITY UPLC H-Class PLUS are designed with the most reliable, highest performing solvent managers and valves on the market – for flexibility, accuracy, and precision of the entire assay.

ACQUITY UPLC 2D LC System ACQUITY UPLC Systems with 2D LC Technology

Specifications

Specifications detailed below are for a typical 2D system with two Binary Solvent Managers (BSM) or Quaternary Solvent Manager (QSM) and BSM, and Flow Through Needle (FTN) Sample Manager. For other available options and additional details, please consult Waters.

Overview

  • Achieve successful 2D UPLC/UHPLC experiments faster, with less troubleshooting and more confidence with ready-to-use configurations
  • Attain best-in-class selectivity and sensitivity with the ACQUITY UPLC Systems and proven columns

Recommended Use: To increase analysis speed, improve sensitivity, selectivity, and perform accurate orthogonal separations.

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Enhanced multidimensional UPLC capabilities

For chromatographers who require additional capabilities to increase their analysis speed, gain sensitivity, selectivity, and perform orthogonal separations, the ACQUITY UPLC systems are ideal. They meet all these needs by controlling multiple columns, valves, and pumps in various configurations.

The resolution, sensitivity, and throughput benefits of the UPLC technology are particularly more important in 2D LC applications. Waters 2D technology solutions for the ACQUITY UPLC M-Class System, ACQUITY UPLC I-Class PLUS, and ACQUITY UPLC H-Class PLUS Systems are purpose-built, from plumbing to software to valve control, providing reproducible, and consistent results.

Advanced functionality to better suit your laboratory's needs

ACQUITY UPLC Systems with 2D Technology can efficiently perform functions such as trapping, heart cutting, parallel column regeneration, at-column dilution, time de-coupled chromatography, and 2D comprehensive chromatography – all enhancing your ability to eliminate unwanted interferences, increase peak capacity, and resolution for characterizing the most complex samples, improve assay ruggedness, and increase the overall analysis speed.

Purposefully designed for 2D LC

The ACQUITY UPLC Column Manager is specifically designed for 2D applications, with the capability to house two columns with different chemistries with independent temperature control for each column, providing versatility for orthogonal separations. The column manager comes standardized with easy-to-access, low-volume, active solvent pre-heaters, resulting in the same efficiency run-to-run, and system-to-system, and supports two 150 mm or four 50 mm length columns.

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The ACQUITY UPLC H-Class PLUS System delivers the flexibility and precision of quaternary or binary solvent blending with the advanced performance expected of UPLC separations: high resolution, sensitivity, and improved throughput.

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The highest performing UPLC System ever engineered. With dramatically decreased dispersion for the highest resolution, rapid injection cycles, and sample throughput with reduced carryover, it's clearly the superior inlet to any mass spectrometer.

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The ACQUITY UPLC Systems with 2D LC Technology bring a new level of precision to the field of analytical chemistry, especially in liquid chromatography (LC). While traditional LC systems are effective for sorting through complex mixtures of substances, they can struggle when faced with samples containing many closely related compounds.

Here's how the ACQUITY UPLC Systems with 2D LC Technology works:

First Dimension Separation: Initially, the sample undergoes separation using one type of chromatography, which might focus on factors like molecular size, polarity, or hydrophobicity. The aim is to break down the sample into its individual components as much as possible.

Comprehensive Sampling: Following the first dimension separation, specific fractions or peaks of interest are chosen for further examination. This selection process can involve heart-cutting, where only a part of the first dimension separation is moved to the second dimension for additional analysis. Alternatively, comprehensive 2D LC entails transferring all fractions from the first dimension to the second for a more thorough analysis.

Second Dimension Separation: In the second dimension, the chosen fractions from the first dimension undergo separation using a different mechanism compared to the first. This adds another layer of resolution, further separating any components that may have co-eluted in the first dimension.

Detection and Analysis: Finally, the separated components are identified and quantified using methods like mass spectrometry or UV-Vis spectroscopy. This enables precise identification and measurement of the compounds present in the sample.

By combining these two separation mechanisms, ACQUITY UPLC Systems with 2D LC Technology offer several benefits:

Enhanced Separation Power: The two-dimensional approach enables higher resolution separation, particularly useful for complex samples with compounds that elute closely together.

Increased Sensitivity: By minimizing interference from co-eluting compounds, 2D LC improves detection and quantification limits for target analytes.

Comprehensive Analysis: Both targeted analysis of specific compounds and exhaustive profiling of complex mixtures are possible through a combination of heart-cutting and comprehensive sampling techniques.

Improved Peak Capacity: The ability to separate a greater number of distinct analytes in a chromatographic system often results in better peak resolution and more accurate identification.

Overall, ACQUITY UPLC Systems with 2D LC Technology serve as invaluable tools for researchers and scientists in various fields such as pharmaceuticals, environmental analysis, metabolomics, and proteomics, where precise characterization of complex mixtures is crucial.

ACQUITY UPLC Systems with 2D LC Technology FAQs

1. What is 2D LC technology?
Two-dimensional liquid chromatography (2D LC) technology enhances liquid chromatography by employing two separate separation dimensions to analyze complex samples. In 2D LC, compounds are separated in the first dimension using one chromatographic method, and further resolved in the second dimension using a different method.
This provides greater resolution, selectivity, and the ability to separate analytes that might co-elute in traditional 1D LC. It is especially beneficial for complex mixtures, offering detailed insights into challenging matrices such as biological samples, food products, and environmental samples.

2. How does the ACQUITY UPLC System with 2D LC Technology differ from traditional LC systems?
The ACQUITY UPLC System with 2D LC Technology expands on traditional LC by integrating two independent chromatographic dimensions in a single workflow. Unlike 1D LC, where separations occur in a single phase, 2D LC allows for orthogonal separations by combining different chemistries or separation mechanisms in the two dimensions. This capability significantly improves resolution, sensitivity, and selectivity for complex or low-abundance analytes. Additionally, 2D LC reduces matrix effects and provides detailed compositional analysis, making it ideal for applications in proteomics, metabolomics, and pharmaceuticals.

3. Can the system handle both targeted and untargeted workflows?
Yes, the ACQUITY UPLC System with 2D LC Technology supports both targeted and untargeted workflows. In targeted analyses, the system provides precise separation and quantification of specific compounds with high selectivity and sensitivity. For untargeted workflows, the dual-dimension approach enables comprehensive profiling of complex mixtures, allowing for the discovery of unknown compounds or subtle variations in the sample composition. This versatility makes the system an excellent choice for method development, biomarker discovery, and comprehensive sample characterization.

4. What are the pressure and flow rate capabilities of the system in 2D LC mode?
The ACQUITY UPLC System with 2D LC Technology is engineered to operate at ultra-high pressures and supports precise flow control in both dimensions. Typically, the first dimension operates at low to moderate flow rates to achieve broad separation, while the second dimension utilizes higher pressures and faster flow rates for rapid resolution.