SFC Prep 150 AP System
Designed for automated UV and/or MS-triggered purification
The SFC Prep 150 AP System is the first and only commercially available preparative SFC system offering automated sample handling, column switching, fraction collection and tracking in an easy-to-use, open bed format.
The SFC Prep 150 AP System provides routine compound purification for drug discovery labs using the MassLynx Software with FractionLynx Application Manager, offering fast separations, high resolution, and high throughput. The inherent benefits of SFC, combined with Waters’ world-renowned service and support provides users with a robust, cost-effective solution to any purification lab.
Specifications
Overview
- Offers automated sample handling, column switching, fraction collection, and tracking in an easy-to-use, open bed format
- Achieve fast separations, high resolution, and high throughput
- Attain routine compound purification for drug discovery labs, using the Waters MassLynx/FractionLynx Application Manager
- Benefit from a robust, cost-effective solution to any purification lab, when combined with world-renowned service and support from Waters
Recommended Use: For drug discovery labs needing fast separations, high resolution, and high throughput in routine compound purification.
Features Header
Automate the purification process
The SFC Prep 150 AP System automates the purification process by linking analytical pre-screen, purification and fraction re-analysis with AutoPurify in an “open access” setting.
SFC systems utilize liquid carbon dioxide (CO2) as its main mobile phase in combination with one or more organic solvents resulting in faster equilibration, lower pressure drops across the column, solvent reduction, and decreased cost per sample. The process is reproducible and applicable to a wide range of compounds relevant in the pharmaceutical, life sciences, chemical materials, food, and environmental markets.
Moving SFC forward
The SFC Prep 150 AP System comes equipped with modules to help with routine compound purification, including:
- QGM Low Pressure mixing Quaternary Gradient Pump: This pump delivers flowrates up to a maximum of 150 mL/min as serves as the co-solvent delivery device for this system.
- P200X CO2 Pump: This is a high-pressure delivery pump having dual stainless-steel heads with a cam driven sapphire piston assembly; self-priming check valves; presser sensor; pressure gauge; brushless motor; and a rupture disc assembly. The pumps design lends itself to control based upon feedback from the pressure sensor and mass flowmeter.
- 3767 Sample Manager: This module provides injection and collection capability in an open bed format. The system includes the fume hood assembly for the 3767 for CO2 management. Injection and collection racks must be ordered for the system. The specific racks required will be determined by your needs and requirements.
- Analytical-2-Prep Column Oven: A thermal control module, the Analytical-2-Prep Column Oven's unique drawer design gives the user unprecedented flexibility, enabling the use of 10 mm, and 20 mm diameter columns simultaneously.
Optional modifier stream injection
The Modifier Stream Injector’s function is to inject one sample at a time directly into the co-solvent flow. This device allows for settable air gaps on either side of the sample, which function as a buffer between the solvent and the sample decreasing dilution. There are two benefits to the MSI:
- The sample is injected into the organic portion of the mobile phase (co-solvent) prior to mixing with CO2. The idea is to mitigate the diluent affect by introducing the sample without affecting the overall strength of the mobile phase, and maintaining the programmed solvent percentages throughout the injection and run. Modifier-stream injection provides improved peak shape and resolution, allowing for larger injection volumes, and higher loading (image 1).
- It allows for Stacked Injections. Stacked injections reduce the time between injection cycles and minimize solvent usage. Stacked injections also significantly improve throughput by utilizing all of the available chromatographic space for continuous separation and purification. Usually, injections are made while an already injected sample is on (or eluting from) the column (image 2).