Resolve your toughest separation challenges with Waters analytical and preparative HPLC columns, which come in 5,800+ column formats, 175+ column configurations, and 21 unique column phases — for excellent performance and column-to-column reproducibility.
Eliminate doubt in UPLC, UHPLC, and HPLC analysis applications with CORTECS Premier Columns, designed to increase sample throughput while providing reproducible data for all analytes.
Experience XBridge HPLC and UHPLC Columns, ideal for methods development at extreme temperatures and mobile-phase pH. Explore XBridge BEH Column chemistries today.
Get superior selectivity and peak shape for basic compounds with XSelect HPLC and UHPLC Columns. Discover our CSH and high-strength silica columns. Visit today.
Meet protein purification needs with Advanced Purification glass columns with silica, polymer or soft gel packings, designed for analytical & preparative LC instruments.
Increase bioanalysis recovery, reproducibility, and enhance LC and LC-MS sensitivity with Waters BioResolve Premier Columns, reversed-phase ion exchange columns designed for bioanalysis applications
Ideal for the isolation, analysis, and characterization of proteins and peptides, BioSuite Columns offer multiple chemistries: C18, cation and anion-exchange, SEC, & Phenyl.
Reliable and robust critical quality attribute analysis of aggregates for Lipids nanoparticles (LNPs), nucleic acids, and viral vectors using GTxResolve columns.
Minimize non-specific adsorption (NSA) inherent to preparative chromatography while yielding high-purity results with MaxPeak Premier OBD Preparative Columns.
Get higher resolution and efficiency for HPLC with Nova-Pak analytical and semi-preparative columns. Discover Nova-Pak C18, C8, Cyano & Phenyl column chemistries.
Achieve high-quality LC-based analyses & lab scale purifications of peptides and proteins with Protein-Pak IEX & HIC Columns, designed for use on HPLC and UPLC systems.
Polymeric reversed-phase columns for basic compounds and applications requiring a broad pH range (2–12). Shodex RSpak Columns chemistries include DE-613 and DS-613.
Get exceptionally high mass loading for both analytical and preparative columns with SunFire HPLC Columns. Discover SunFire Silica, C8, and C18 columns.
Safeguard analytical HPLC, UHPLC, and UPLC columns from the effects of damaging particulates and chemical contaminates with VanGuard Cartridges and Pre-columns.
Discover unique separation selectivities with Waters Spherisorb Columns, the most widely referenced products for analytical and preparative column chromatography.
Experience XBridge Premier UHPLC Columns, designed to operate under extreme temperatures and mobile-phase pH while mitigating unwanted analyte/surface interactions.
Perform low and high pH drug discovery, method development and purification separations with XTerra hybrid particle technology HPLC columns. Learn more.
Separate acidic, basic, and neutral compounds with µBondaPak and BondaPak Columns. These HPLC columns are available in phenyl, C18, cyano and NH2 column chemistries.
Get reliable normal-phase chromatography separations with non-bonded, silica-based μPorasil and Porasil Columns. Research µPorasil/Porasil HPLC Column options.
HPLC Columns
Waters offers advanced high performance liquid chromatography (HPLC) columns designed to provide exceptional chromatography, guaranteeing consistent and reproducible results. Available in reversed phase and hydrophilic interaction liquid chromatography (HILIC) variants, these columns cater to different compound types.
Reversed phase columns excel in separating nonpolar or hydrophobic compounds, utilizing a hydrophobic stationary phase that interacts based on hydrophobicity. This interaction enables precise separation of molecules with varying hydrophobic characteristics, resulting in well-resolved chromatograms and accurate quantification.
Hydrophilic interaction liquid chromatography (HILIC) columns are tailored for polar and hydrophilic compounds, employing a hydrophilic stationary phase to retain and separate these analytes. This is achieved by exploiting interactions between their hydrophilic functional groups and the stationary phase. This approach is particularly useful for compounds not retained well on conventional reversed phase columns.
Waters' columns incorporate advanced materials and technologies, ensuring precision, reliability, and reproducibility in chromatography. Engineered for consistent performance across diverse analyses, these columns instill confidence in researchers, yielding accurate and repeatable outcomes across applications such as pharmaceuticals, environmental monitoring, and food and beverages.
Address challenging separations using Waters' analytical and preparative HPLC columns, available in over 5,800 column formats, 175 configurations, and 21 phases. Achieve exceptional performance and reproducibility across columns.
FAQs about HPLC Columns
How do I select the right HPLC column for my application?
Selecting the right HPLC column involves considering the chemical properties of your analytes, such as molecular weight, polarity, and pKa. Choose a stationary phase that complements your analytes; for example, Waters offers various phases like C18, C8, and phenyl. Smaller particle sizes (e.g., sub-2 µm) provide higher resolution and efficiency but require higher pressures. Pore size should match your analytes, with larger pores for proteins and smaller pores for small molecules. Also, consider column dimensions based on your separation needs and ensure compatibility with your HPLC system and solvents.
What is the difference between UPLC and HPLC columns?
UPLC (Ultra-Performance Liquid Chromatography) columns use sub-2 µm particles, while HPLC (High-Performance Liquid Chromatography) columns use particles that are 3 µm or larger. UPLC columns offer higher resolution, sensitivity, and speed due to the smaller particle size and can operate at much higher pressures (up to 15,000 psi) compared to HPLC systems (up to 6,000 psi). This results in sharper peaks and better separation efficiency.
How do Waters' BEH C18 columns differ from other C18 columns?
Waters' BEH (Ethylene Bridged Hybrid) C18 columns offer exceptional chemical stability, operating under a wide pH range (typically pH 1-12). The BEH technology enhances durability, providing longer column lifetimes and consistent performance under harsh conditions. These columns deliver superior peak shapes, resolution, and sensitivity, especially in UPLC applications. BEH C18 columns are versatile and suitable for small molecule analysis, peptide mapping, and protein separations, with optimized particle design ensuring high efficiency and reproducibility.
Eliminate doubt in UPLC, UHPLC, and HPLC analysis applications with CORTECS Premier Columns, designed to increase sample throughput while providing reproducible data for all analytes.
Discover XSelect Premier UHPLC Columns designed to improve peak shape and maximize selectivity while mitigating unwanted analyte/surface interactions.
Maximize resolution and peak capacity for LC separations with CORTECS solid-core columns in 1.6 and 2.7 µm across 7 column chemistries.
Experience XBridge HPLC and UHPLC Columns, ideal for methods development at extreme temperatures and mobile-phase pH. Explore XBridge BEH Column chemistries today.
Improve chromatographic performance, retention, and stability for polar compounds with Atlantis HPLC Columns. Discover Atlantis C18 and HILIC column chemistries now.
Get superior selectivity and peak shape for basic compounds with XSelect HPLC and UHPLC Columns. Discover our CSH and high-strength silica columns. Visit today.
Meet protein purification needs with Advanced Purification glass columns with silica, polymer or soft gel packings, designed for analytical & preparative LC instruments.
Streamline analytical challenges associated with mAb characterizations and associated biotherapeutic drug separations with BioResolve Columns.
Increase bioanalysis recovery, reproducibility, and enhance LC and LC-MS sensitivity with Waters BioResolve Premier Columns, reversed-phase ion exchange columns designed for bioanalysis applications
Ideal for the isolation, analysis, and characterization of proteins and peptides, BioSuite Columns offer multiple chemistries: C18, cation and anion-exchange, SEC, & Phenyl.
Separate and isolate peptides, proteins, and natural products with Delta-Pak HPLC Columns for large molecule separations.
Reliable and robust critical quality attribute analysis of aggregates for Lipids nanoparticles (LNPs), nucleic acids, and viral vectors using GTxResolve columns.
Minimize non-specific adsorption (NSA) inherent to preparative chromatography while yielding high-purity results with MaxPeak Premier OBD Preparative Columns.
Get higher resolution and efficiency for HPLC with Nova-Pak analytical and semi-preparative columns. Discover Nova-Pak C18, C8, Cyano & Phenyl column chemistries.
Achieve high-quality LC-based analyses & lab scale purifications of peptides and proteins with Protein-Pak IEX & HIC Columns, designed for use on HPLC and UPLC systems.
Achieve high retention and high resolution of polar compounds with non-endcapped Resolve Columns available in C18, C8 and Cyano column chemistries.
Polymeric reversed-phase columns for basic compounds and applications requiring a broad pH range (2–12). Shodex RSpak Columns chemistries include DE-613 and DS-613.
Get exceptionally high mass loading for both analytical and preparative columns with SunFire HPLC Columns. Discover SunFire Silica, C8, and C18 columns.
Get predictable analytical performance and ensure batch-to-batch reproducibility with high purity silica-based Symmetry HPLC Columns.
Safeguard analytical HPLC, UHPLC, and UPLC columns from the effects of damaging particulates and chemical contaminates with VanGuard Cartridges and Pre-columns.
Analyze insulin products for impurities with Waters Insulin HMWP Gel Filtration HPLC Columns. Learn about insulin gel filtration column packing now.
Get superior peak identification and quantitation for Polyaromatic Hydrocarbons (PAHs) compounds with Waters PAH C18 HPLC Columns.
Discover unique separation selectivities with Waters Spherisorb Columns, the most widely referenced products for analytical and preparative column chromatography.
Experience XBridge Premier UHPLC Columns, designed to operate under extreme temperatures and mobile-phase pH while mitigating unwanted analyte/surface interactions.
Perform low and high pH drug discovery, method development and purification separations with XTerra hybrid particle technology HPLC columns. Learn more.
Separate acidic, basic, and neutral compounds with µBondaPak and BondaPak Columns. These HPLC columns are available in phenyl, C18, cyano and NH2 column chemistries.
Get reliable normal-phase chromatography separations with non-bonded, silica-based μPorasil and Porasil Columns. Research µPorasil/Porasil HPLC Column options.