Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
The innovative 96-well Oasis HLB µElution plate is designed for SPE cleanup and analyte enrichment of sample volumes ranging from 10 µL to 375 µL. Each well of the µElution plate contains 2 mg of the Oasis HLB sorbent, which is a universal polymeric reversed-phase sorbent for extraction of a wide range of acidic, basic, and neutral compounds from various matrices using a simple, generic protocol.
The innovative features of Oasis µElution plate enabled elution in only 25 µL, resulting in sensitive, robust, reproducible results without evaporation and reconstitution. A 15-fold sample enrichment is possible with this plate. The Oasis HLB µElution plate is compatible with most liquid-handling robotic systems for automated, high throughput SPE.
Designed to simplify and improve SPE clean-up and analyte enrichment, the 96-well Oasis HLB µElution plate can handle sample volumes that range from 10 µL to 375 µL. Each of the 96 wells in the µElution plate contains 2 mg of Oasis HLB sorbent. This universal polymeric reversed-phase sorbent allows for extraction of acidic, basic, and neutral compounds of many varieties and many matrices through a simplified and generic protocol. The Oasis HLB sorbent is made from a specific ratio of two monomers, providing enhanced retention of polar analytes and superior reverse-phase capacity.
Achieve results that are not only sensitive, but also robust and reproducible, with the innovative Oasis HLB 96-well µElution plate, which enables elution on only 25 µL. This is able to occur without evaporation or reconstitution. This plate makes possible a 15-fold increase in sample concentration.
Create superior retention capacity, allowing a wider polarity spectrum of analytes, even in cases where the sorbent bed becomes dry during conditioning and sample loading due to the water-wettable sorbents in the Oasis HLB 96-well µElution plate. You will eliminate the need for repeat preparation because of the rugged and robust SPE methods offered through this patented sorbent. Your processes become more reproducible as more analytes are retained due to the heightened retention capacity.
Enjoy automated, high volume throughput SPE with the Oasis HLB 96-well µElution plate, which is compatible with the majority of liquid-handling robotic systems. Combining the Waters patented plate design, proven chemistries of the Oasis line, and straightforward protocols, the Oasis HLB 96-well µElution plate will help your lab to deliver high analyte recovery and clean extracts, even in volumes as low as 25 µL.
1. What is the expected sample volume and flow rate for this plate?
The typical sample volume for the Oasis HLB 96-well µElution Plate can vary depending on the specific application and analyte concentration. However, common sample volumes range from 100 µL to 1 mL.
The flow rate should be optimized to balance recovery and throughput. A slower flow rate can improve recovery, especially for weakly retained analytes, while a faster flow rate can increase throughput. Typically, flow rates between 0.5 and 2 mL/min are used.
2. What is the plate's stability and lifetime under typical operating conditions?
The stability and lifetime of the Oasis HLB 96-well µElution Plate can be influenced by several factors, including storage conditions, temperature, and the types of solvents used.
Under proper storage conditions (cool, dry, and sealed), the plate can maintain its performance for several months. However, repeated exposure to high temperatures, organic solvents, and strong acids or bases can degrade the sorbent and reduce its performance.
3. What is the sorbent material and particle size of this plate?
The Oasis HLB 96-well µElution Plate utilizes a hydrophilic-lipophilic balance (HLB) sorbent material. This mixed-mode sorbent effectively retains both polar and non-polar compounds, making it suitable for a wide range of analytes. The sorbent particles in this plate have a size of 30 µm, which provides a balance between efficient mass transfer and sufficient capacity for analyte retention.
4. How does the selectivity of this plate compare to other HLB plates?
The Oasis HLB 96-well µElution Plate offers excellent selectivity for a wide range of analytes due to its unique HLB sorbent. Compared to other HLB plates, this plate often demonstrates superior retention and recovery for both polar and non-polar compounds. However, the specific selectivity can vary depending on factors such as sample matrix, analyte properties, and method parameters.
What is elution?
Elution refers to a process in analytical or organic chemistry, in which one material is extracted from another via a wash using solvent. The volume of eluent, or the liquid solvent, needed to result in elution is referred to as the elution volume. The eluate is the analyte material that emerges from the process.
What is SPE?
SPE, an abbreviation for solid-phase extraction, involves the separation of dissolved or suspended compounds in a liquid mixture based on their distinctive chemical and physical properties. This process is frequently employed in laboratories to concentrate or purify samples, thereby enhancing the accuracy of analysis.
