ACQUITY UPLC & ACQUITY Premier PDA eLambda Detectors

ACQUITY UPLC & ACQUITY Premier PDA eLambda Detectors

PDA detection for spectral exposure optimization

PDA detection for spectral exposure optimization

Getting accurate and complete spectral information to make better decisions about your analysis can be a challenge. The ACQUITY UPLC and ACQUITY Premier Photodiode Array (PDA) eλ Detectors enable a laboratory to detect and quantify lower concentrations of sample analytes as well as compare spectra across the ultraviolet and visible wavelengths, allowing analyses encompassing a broader concentration range. 

The detectors meet multiple UPLC-MS detection strategy requirements and provide the ability to map low levels of compounds and determine trace impurity levels within a compound’s peak.

ACQUITY UPLC PDA eλ Detector. ACQUITY UPLC & ACQUITY Premier PDA eLambda Detectors

Specifications

Wavelength range

190 to 800 nm

Wavelength accuracy

±1 nm

Linearity range

Deviation at 2.0 AU ≤5%, propylparaben, at 257 nm

Optical resolution

1.2 nm

Digital resolution

1.2 nm/pixel

Baseline noise

±3 μAU, 230 nm, 2 Hz, 2 s, wavelength compensation 310 to 410, WET (2D channel)

Drift

≤1.0 x 10-3/AU/hour/°C

Sampling rate

Up to 80 points/s

Light source

Prealigned, intelligent technology Deuterium lamp

Dispersion volume

≤0.8 μL (Analytical flow cell); ≤1.5 μL (High sensitivity flow cell)

Flow cell design

Light-guiding UPLC intelligent technology flow cell

Path length

10 mm (analytical flow cell)

Flow cell volume

500 nL (analytical flow cell)

Wetted materials

316 stainless-steel, fused silica, Teflon AF, PEEK (analytical flow cell)

Pressure limit

1000 psi (all flow cells)

Flow cell options

Analytical: 500 nL, 10 mm path length

High sensitivity: 2400 nL, 25 mm path length

High throughput: 135 nL, 3 mm path length

Stainless-steel: 1500 nL, 5 mm path length

Titanium: 1500 nL, 5 mm path length

ACQUITY Premier PDA Flow Cell, HPS

Titanium: 5 mm, AP

Physical specifications

Width: 34.3 cm (13.5 inches)

Height: 19.4 cm (7.6 inches)

Depth: 60.7 cm (23.9 inches)

Overview

  • Quantitate trace impurities with high optical and digital resolution
  • Library matching and peak purity assessment for increased confidence in compound identification
  • Data rates of up to 80 Hz, noise specifications of ±3 µAU, and reproducible integration allows for detection of peaks generated by fast UPLC/PDA separations
  • Efficient light transmission and low noise characteristics for maximum sensitivity
  • Simultaneous 2D and 3D operation in either Empower or MassLynx Software
  • Lamp intensity optimized by unique software to maintain minimal noise levels across the entire deuterium spectrum for the life of the lamp

Recommended Use: Detect and quantify lower concentrations of sample analytes and compare spectra across a broad wavelength range.

Features Header

Features Header

Unmatched sensitivity with enhanced flexibility

The Waters ACQUITY UPLC and ACQUITY Premier PDA eλ Detectors are specifically designed to complement Performance UHPLC/UPLC Systems. These detectors have data rates up to 80 Hz, noise specifications of ±3 μAU, and sensitivity that is unmatched by any other photodiode array detector on the market. Further, enhanced software control provides flexibility for simultaneous 2D and 3D operations in Empower or MassLynx software.

Optimal performance across the entire spectrum range

The expanded wavelength range of the ACQUITY UPLC and ACQUITY Premier PDA eλ Detectors provides for highly sensitive spectral information in the visible region. Noise performance in lower energy regions of the deuterium spectrum is improved while maintaining optimal performance across the entire range.

Resources

Documents

Documents

Support

Support

What do you want to do?

What do you want to do?
Need Help?

Related

ACQUITY Premier System
Experience the ultimate in sensitivity, reproducibility, and speed. The ACQUITY Premier LC-MS provides the best sensitivity and peak shape for analytical separations.

ACQUITY UPLC H-Class PLUS System
The ACQUITY UPLC H-Class PLUS System delivers the flexibility and precision of quaternary or binary solvent blending with the advanced performance expected of UPLC separations: high resolution, sensitivity, and improved throughput.

ACQUITY UPLC I-Class PLUS System
The highest performing UPLC System ever engineered. With dramatically decreased dispersion for the highest resolution, rapid injection cycles, and sample throughput with reduced carryover, it's clearly the superior inlet to any mass spectrometer.

ACQUITY UPLC Columns
Discover how to achieve increases in LC efficiency, resolution, sensitivity, accuracy, speed, and reliability with ACQUITY sub-2 μm UPLC particle columns.
Back To Top Back To Top

The ACQUITY UPLC Premier PDA eLambda Detectors are cutting-edge tools for analyzing compounds in various substances. They use advanced technology called Ultra-Performance Liquid Chromatography (UPLC) to separate compounds quickly and accurately. These detectors have photodiode arrays (PDAs) at their core, which are highly advanced sensors that measure light absorption across different wavelengths simultaneously. This allows for detailed information about the compounds being analyzed in real-time.

The "eLambda" feature integrated into the ACQUITY UPLC Premier PDA detectors plays a pivotal role in enhancing the accuracy of wavelength measurements, thereby significantly aiding in the precise identification and quantification of compounds, even when they exist in minuscule concentrations. This technological advancement holds immense significance for researchers and analysts dealing with intricate mixtures, as it empowers them to discern impurities and measure target compounds with unprecedented precision.

Across a multitude of industries including pharmaceuticals, biotechnology, environmental analysis, and food and beverage testing, these detectors serve as indispensable tools. By facilitating higher levels of accuracy and efficiency in analytical procedures, they ensure that laboratories can produce results of the utmost reliability. Researchers and analysts can place full trust in the data generated by these detectors, knowing that they provide a robust foundation upon which critical decisions can be made.

In essence, the ACQUITY UPLC Premier PDA eLambda Detectors present a comprehensive solution for chromatographic analysis. Their incorporation of advanced features not only elevates the quality of results but also bolsters the confidence with which conclusions are drawn. As laboratories continue to rely on chromatography for crucial investigations and assessments, these detectors helps in achieving advancements and breakthroughs across various scientific and industrial domains.

FAQs About ACQUITY UPLC & ACQUITY Premier PDA eLambda Detectors

1. What is the detection range of the ACQUITY Premier PDA eLambda Detector?
The ACQUITY Premier PDA eLambda Detector provides a broad detection range, covering wavelengths from 190 to 800 nanometers. This extensive range allows for accurate detection of a wide variety of compounds, including those that absorb in both UV and visible regions. The eLambda Detector's wide detection capability supports complex multi-component analysis, offering high sensitivity for low-concentration analytes and precision for high-absorbance compounds. This range makes the detector highly versatile and suitable for applications across pharmaceuticals, environmental testing, food safety, and life sciences.

2. What sample types are compatible with the ACQUITY UPLC system?
The ACQUITY UPLC system is compatible with a wide range of sample types, including small molecules, peptides, proteins, oligonucleotides, and biopharmaceuticals. It is designed to handle complex biological, environmental, food, and chemical samples with minimal interference and high precision, and the system’s flexibility in supporting diverse sample matrices makes it ideal for research in pharmaceuticals, clinical trials, environmental analysis, and industrial applications. The ACQUITY UPLC system also offers specialized columns and solvent compatibility, allowing for effective separation and accurate results across various challenging sample types.

3. What software platforms are compatible with the ACQUITY Premier PDA eLambda Detector?
The ACQUITY Premier PDA eLambda Detector is compatible with several major software platforms, including Waters’ Empower and MassLynx. These platforms offer robust data analysis tools, helping users perform complex analyses, automate workflows, and ensure regulatory compliance. Empower software, for instance, is especially valued for its user-friendly interface, secure data management, and extensive reporting options. MassLynx, meanwhile, is ideal for advanced mass spectrometry applications and integrates well with the eLambda Detector for enhanced data acquisition and processing. This compatibility enables labs to maximize efficiency and maintain data integrity across diverse analytical workflows.