Atlantis Columns

Atlantis Columns

Reversed-phase and HILIC HPLC Columns for superior polar compound retention

Reversed-phase and HILIC HPLC Columns for superior polar compound retention

Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.

Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures. 

Atlantis HPLC Column Family Atlantis Columns
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Unwanted interactions between analytes and metal ions?

Atlantis Premier Columns with MaxPeak HPS technology mitigate interactions with stainless-steel column hardware.

Specifications

Overview

  • Retains and separates small, water-soluble polar organic compounds
  • Compatible with 100% aqueous mobile phases
  • Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents
  • Long column lifetimes using low-pH mobile phases
  • Fully scalable in 3, 5, and 10 μm particle sizes

Recommended Use: For the reproducible retention and separation of a broad range of polar and non-polar compounds.

Atlantis Column Characteristics

Atlantis Column Characteristics
Atlantis T3 Columns
  • Superior retention of polar compounds
  • Compatible with 100% aqueous mobile phases
  • Exceptional column lifetime at low pH
  • Universal reversed-phase column for open access environments

Tri-functionally C18 bonding designed to improve column lifetimes and deliver exceptional reproducible performance for an expansive range of compound polarities.

Atlantis dC18 Columns
  • Superior retention of polar compounds
  • Compatible with 100% aqueous mobile phases
  • Reversed-phase columns

Designed to deliver exceptional reproducible performance for both polar and non-polar molecules.

Atlantis HILIC Columns
  • Excellent for the retention of very polar, basic, water-soluble analytes
  • Specifically designed and tested for HILIC separations
  • Employ highly volatile (>80% organic) mobile phases
  • Hydrophilic Interaction Chromatography (HILIC) silica columns

Ideal for mass spectrometry (MS) response and sensitivity.

Ideal for diverse analyte mixtures

When faced with analyzing several compounds across a broad range of polarity, few options will adequately retain polar species without excessively retaining less polar components. Atlantis T3 Columns are a universal, silica-based, reversed-phase C18 line of HPLC Columns, designed to deliver exceptional performance and give you the ability to retain and separate small, water-soluble polar organic compounds.

Seamless transfer across technology platforms

Based on different particle substrates, Waters Atlantis T3 and HSS T3 Columns enable labs to achieve similar selectivity and retention across HPLC and UPLC technology platforms, enabling ease of method transfer across an organization. 

Polar compound retention without ion-pairing reagents

Eliminating ion-pairing reagents improves detection limits, method reproducibility, and robustness, while reducing instrument maintenance from harsh mobile-phase environments. Atlantis HILIC Silica Columns retain very polar, organic molecules that cannot be retained using reversed-phase (RP) methods. Unlike the highly aqueous mobile phases required for polar retention in reversed-phase separations, Atlantis HILIC Silica Columns increase retention using volatile organic-based mobile phases, ideal for mass spectrometry (MS) response and sensitivity.

Scale from analytical to prep with ease

Atlantis T3 and HILIC Silica Prep OBD (Optimum Bed Density) Columns offer analytical column performance in preparative chromatography formats to deliver a variety of advantages, including improved bed stability, higher efficiency, longer column lifetime, and increased loadability.

Stay compliant with smart column options

Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (3 µm particles) used with Alliance iS HPLC System that is optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.

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Related

Atlantis Premier Columns
Increase polar analyte retention over a wider pH range and mitigate unwanted analyte/surface interactions with Atlantis Premier Columns.

VanGuard Column Protection
Prolong analytical column lifetime while maintaining chromatographic performance with VanGuard Pre-columns and cartridges.

Sample Vials
Reduce failed assays with Waters glass and plastic autosampler vials and high sample recovery vials - certified/tested by HPLC, GC, and MS.

Quality Control Reference Materials
Ensure peak LC and LC-MS column and system performance by benchmarking with Waters Quality Control Reference Materials, a portfolio of ready-to-use standards and mixes.

Offers and Discounts

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Atlantis Columns

The Waters Atlantis Columns are designed to tackle one of the most persistent challenges in chromatography: the retention and separation of polar analytes. Polar compounds, due to their inherent properties, often elute early in traditional reversed-phase (RP) chromatography, sometimes even in the void volume of the column, leading to poor retention and inadequate separation. This challenge is effectively addressed by Atlantis Columns, which have been engineered to retain and separate polar analytes efficiently under both reversed-phase and HILIC (Hydrophilic Interaction Liquid Chromatography) conditions. This makes them indispensable tools for achieving quick, accurate, and reproducible quantification with enhanced retention and sensitivity.

Atlantis Columns are available in three unique chemistries: T3, dC18, and unbonded HILIC, each tailored to provide exceptional performance for a diverse range of applications:

  • T3 chemistry offers balanced retention and separation for both polar and nonpolar compounds, making it a versatile choice for complex mixtures.
  • T3dC18 chemistry, with its enhanced hydrophobicity, delivers strong retention of nonpolar compounds while maintaining excellent performance for polar analytes.
  • T3Unbonded HILIC chemistry is specifically designed for the retention of highly polar, small organic compounds, making it particularly effective in applications where traditional RP methods fall short.

    One of the most significant advantages of the Atlantis Columns is their ability to work seamlessly with 100% aqueous mobile phases. This capability is crucial for the analysis of polar compounds, which are often highly soluble in water. Unlike many other columns that require the use of ion-pairing reagents to retain polar analytes, Atlantis Columns eliminate this need, thus improving detection limits and enhancing method reproducibility and robustness. This not only simplifies the chromatographic process but also contributes to the more reliable and consistent results required in research and routine analysis settings.

    The long column lifetimes offered by Atlantis Columns are another standout feature. These columns are engineered to withstand the harsh conditions often encountered in low-pH mobile phases, which can rapidly degrade lesser columns. The durability of Atlantis Columns ensures that they maintain their performance over extended periods, reducing the frequency of replacements and, consequently, lowering operational costs. This longevity, combined with their robust construction, makes Atlantis Columns a cost-effective choice for laboratories that demand high performance and reliability.

    Atlantis Columns are also fully scalable, available in particle sizes of 3, 5, and 10 μm. This scalability allows users to tailor their column selection to specific application requirements, whether for method development, high-throughput analysis, or detailed investigative studies. The ability to choose the appropriate particle size ensures that you can achieve optimal separation efficiency and resolution, regardless of the complexity of the sample matrix.

    In addition to their technical advantages, Atlantis Columns also offer a high degree of versatility, making them suitable for a wide range of applications. Whether you are working with pharmaceutical compounds, environmental samples, or complex biological matrices, Atlantis Columns provide the retention, separation, and stability needed to achieve high-quality results.  Their balanced retention capabilities make them particularly useful for applications involving broad analyte mixtures, where both polar and nonpolar compounds need to be effectively separated and quantified.

    Waters Atlantis Columns are an essential asset for any chromatographer seeking to overcome the challenges associated with polar compound retention and separation. Their advanced chemistries, compatibility with aqueous mobile phases, extended lifetimes, and scalability make them a powerful and versatile tool for achieving superior chromatographic performance.

    FAQs about Atlantis Columns

    1.Are Atlantis columns compatible with both HPLC and UHPLC systems?
    Yes, Atlantis columns are compatible with both HPLC and UHPLC systems. They are designed to withstand the higher pressures and flow rates associated with UHPLC while maintaining excellent performance and durability.

    2.What is the recommended column temperature for Atlantis columns?
    The recommended column temperature for Atlantis columns can vary depending on the specific application and the desired separation conditions. However, a typical operating temperature range is between 25°C and 60°C. Increasing the column temperature can often improve peak resolution and reduce retention times, but it is important to consider the stability of the analytes and the stationary phase.

    3.Are Atlantis columns available with different stationary phases (e.g., C18, C8, HILIC)?
    Yes, Atlantis columns are available with a variety of stationary phases, including C18, C8, and HILIC. This allows for versatility in chromatographic separations, enabling the analysis of a wide range of analytes with different polarities.
    C18 stationary phase is ideal for the separation of nonpolar and moderately polar compounds, while the C8 stationary phase offers intermediate selectivity between C4 and C18, making it suitable for a broader range of analytes. The HILIC stationary phase is designed for the analysis of polar and hydrophilic compounds, often used in conjunction with hydrophilic mobile phases.