XSelect Columns

XSelect Columns

Achieve unmatched reproducibility with a wide range of selectivity

Achieve unmatched reproducibility with a wide range of selectivity

Quickly and easily develop robust methods compatible with all modern chromatographic detection modes and transfer them from lab to lab with the XSelect Column family.

Designed to help you achieve reproducible and highly selective chromatographic separations, the XSelect Column family features two base particles technologies, Charged Surface Hybrid (CSH) and High Strength Silica (HSS). With XSelect Columns, method development scientists like you can achieve a wide range of selectivities across all application areas, without compromising performance.

XSelect HPLC & UHPLC Column Family XSelect Columns
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Unwanted interactions between analytes and metal ions?

XSelect Premier Columns with MaxPeak HPS technology mitigate interactions with stainless-steel column hardware.

Specifications

Overview

  • Designed for selectivity and stability at low pH
  • Superior peak shape for basic compounds
  • Highest analyte mass loading available
  • Excellent batch-to-batch reproducibility
  • Ideal for rapid method development
  • CSH Column chemistries: C18, Fluoro-Phenyl (PFP), and Phenyl-Hexyl, available in 2.5 XP, 3.5, 5 μm analytical and 10 μm OBD preparative sizes
  • HSS Column chemistries: HSS C18, C18 SB, and T3, available in 2.5 XP, 3.5, and 5 μm particle sizes
  • Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using XSelect stationary phases

Recommended Use: For improving selectivity, peak shape, and retentivity in methods development.

XSelect CSH Column Characteristics

XSelect CSH Column Characteristics
XSelect CSH-C18 Columns
  • Alternative selectivity to existing C18 columns
  • Low anion exchange
  • High efficiency
  • Excellent pH stability
  • Ideal for improved peak shape for bases with low pH mobile phase
  • General purpose hybrid column
XSelect CSH Phenyl-Hexyl Columns
  • Alternative selectivity for aromatic compounds
  • Medium anion exchange
  • High efficiency
  • Excellent pH stability
  • Ideal for improved peak shape for bases with low pH mobile phase
  • General purpose hybrid column
XSelect CSH Fluoro-Phenyl (PFP) Columns
  • Alternative selectivity for acids and bases
  • High anion exchange
  • High efficiency
  • Excellent pH stability
  • Ideal for improved peak shape for bases with low pH mobile phase
  • General purpose hybrid column

XSelect HSS Column Characteristics

XSelect HSS Column Characteristics
XSelect HSS-C18 Columns
  • High efficiency for acids, bases, neutrals
  • Excellent low pH stability
  • General purpose silica columns
  • High retention
XSelect HSS T3 Columns
  • Excellent retention for polar analytes
  • 100% water wettable
  • High retention
  • High efficiency
  • Excellent low pH stability
XSelect HSS C18 SB Columns
  • Alternative selectivity for bases
  • Increased cation exchange at mid pH
  • General purpose silica columns
  • High retention
  • High efficiency
  • Excellent low pH stability
XSelect HSS PFP Columns
  • Alternative selectivity for polar bases
  • Alternative selectivity for aromatic compounds
  • General purpose silica columns
  • High retention
  • High efficiency
  • Excellent low pH stability
XSelect HSS CN Columns
  • Stable and reproducible cyano chemistry
  • Alternative selectivity and increased retention for polar compounds
  • General purpose silica columns
  • High retention
  • High efficiency
  • Excellent low pH stability

XSelect HPLC and UHPLC Application-Specific Column Characteristics

XSelect HPLC and UHPLC Application-Specific Column Characteristics
XSelect CSH C18 Peptide Columns
  • Outstanding peak capacities with formic acid for MS-based applications
  • Excellent performance with TFA for optical based applications
  • Less dependence on TFA or FA to achieve optimal peak capacity
  • Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities
  • At low pH with the positive charge on CSH, you get less secondary interactions with the particle

Try XSelect CSH Peptide Columns

XSelect HSS T3 Peptide Columns
  • Separations that require silica-based selectivity
  • Separations that necessitate increased peptide retention
  • Ideally suited for small, hydrophilic peptides

Try XSelect HSS Peptide Columns

Increase selectivity across compounds

Using selectivity and retentivity to influence chromatographic behavior is common in method development. With the choice of phases in the XSelect HPLC and UHPLC family, you can achieve selectivity and retention across a wide variety of compounds by using different base particles.

The XSelect HSS and CSH particles provide enhanced selectivity for acidic, alkaline and neutral compounds. The XSelect CSH Phenyl-Hexyl Column places more emphasis on reversed-phase interactions compared to secondary Lewis-base interaction. The XSelect HSS C18 SB Column was engineered to provide enhanced retention for basic polar compounds using low-pH mobile phases. When compared to a standard C18 phase, XSelect HSS T3 Columns provide increased retention for polar compounds.

Powerful tools for your method development

The scalability and versatility of XSelect CSH Columns enables you, the isolation and purification scientist, to use them as powerful method development tools. The hybrid materials used to create the CSH stationary phases are known industry-wide for being the most pressure-tolerant columns on the market. Rugged performance combined with Optimum Bed Density (OBD) packing technology produces columns that have exceptional column lifetimes under a wide range of operating conditions. With the columns’ scalable design, you can choose from larger to smaller and narrower preparative columns to reduce solvent consumption and fraction volumes.

Achieve flexibility in method development

Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.

Stay compliant with smart column options

Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (3 µm particles) used with Alliance iS HPLC System that is optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.

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Related

VanGuard Column Protection
Prolong analytical column lifetime while maintaining chromatographic performance with VanGuard Pre-columns and cartridges.

XSelect Premier Columns
Discover XSelect Premier HPLC and UHPLC Columns designed to improve peak shape and maximize selectivity while mitigating unwanted analyte/surface interactions.

Quality Control Reference Materials
Ensure peak LC and LC-MS column and system performance by benchmarking with Waters Quality Control Reference Materials, a portfolio of ready-to-use standards and mixes.

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XSelect Columns

Waters' XSelect Columns represent a breakthrough in achieving high reproducibility and selectivity for method development in the world of chromatography. By incorporating advanced particle technologies such as Charged Surface Hybrid (CSH) and High Strength Silica (HSS), XSelect Columns are specifically engineered to facilitate quick and efficient method development, ensuring consistent performance across various laboratories.

XSelect Columns leverage two foundational technologies to offer unmatched selectivity in chromatography. The Charged Surface Hybrid (CSH) technology is tailored for superior peak shapes and high analyte mass loading, which is particularly effective for basic compounds. Conversely, High Strength Silica (HSS) enhances stability and performance at low pH, broadening the application range to include acidic, alkaline, and neutral compounds. This dual approach allows scientists to tackle a diverse array of chromatographic challenges with ease.

XSelect Columns are renowned for their scalability and versatility, attributes that are critical in method development and transfer. The columns are designed to be compatible with all modern chromatographic detection modes and can be seamlessly transferred from one lab to another without loss of performance. This universality is particularly valuable in environments where methods need to be developed quickly and implemented across multiple sites.

Waters' commitment to innovation is further demonstrated in the longevity of XSelect Columns. Optional VanGuard Cartridges, utilizing the same stationary phases, can significantly extend column life. Moreover, the columns come equipped with smart technology options such as the eCord and eConnect systems. These devices are instrumental for tracking column usage, facilitating compliance in regulated laboratories, and ensuring consistency in performance.

The range of available chemistries within the XSelect family is vast, including C18, Fluoro-Phenyl (PFP), and Phenyl-Hexyl on the CSH side, and HSS C18, C18 SB, and T3 on the HSS side. These options are available across different particle sizes from 2.5 XP to 10 μm OBD, catering to both analytical and preparative needs. This variety ensures that every chromatographer can find the exact column required for their specific separation task.

For laboratories that adhere to stringent regulatory standards, XSelect Columns are an ideal choice. The smart column options, like the eCord data recording device and the eConnect electronic tags, provide essential data management capabilities. These features allow easy access to comprehensive column history and facilitate effective troubleshooting and method optimization, which is crucial for maintaining high throughput and operational efficiency.

Waters' XSelect Columns set a new standard in chromatographic separations, combining cutting-edge technology with exceptional ease of use. By offering a wide range of selectivities, superior batch-to-batch reproducibility, and robust method development tools, XSelect Columns empower chromatographers to advance their research and development efforts. Whether in analytical research, drug discovery, or quality control, these columns ensure that every separation is precise, reproducible, and scalable, making them a cornerstone in modern chromatographic laboratories.